Bond S L, Wigle M R, Singh S M
Department of Zoology, University of Western Ontario, London, Canada.
Alcohol Clin Exp Res. 1991 Mar;15(2):304-7. doi: 10.1111/j.1530-0277.1991.tb01874.x.
The genotype-dependent response of mice to ethanol has been well documented. Cytosolic acetaldehyde dehydrogenase (ALDH-2) increases in some strains while decreasing in others with ethanol treatment. Further work suggests that the mRNA for ALDH-2 (Ahd-2 mRNA) levels are altered following ethanol feeding in a strain-dependent fashion. This report identifies differences in Ahd-2 at the genomic DNA level among different strains of mice. Restriction fragment length polymorphisms (RFLPs) associated with the Ahd-2 locus were found for the restriction enzymes EcoRI, HindIII, Pst I and Rsa I. The mouse strains included in this study could be categorized into two groups based on their overall Ahd-2 associated DNA banding patterns. Strains C57BL/6J, C57BL/6J*, C57BL/10J and BALB/c form group 1 while strains C3H/HeJ, C3H/HeSnJ, 129/ReJ, Csb, SW and DBA/2J form group 2. With the exception of BALB/c, group 1 represents alcohol preferring strains while group 2 are alcohol avoiding strains. Additional work will be required to determine the physiological significance (if any) of these RFLPs and their possible relationship to ethanol preference and avoidance.
小鼠对乙醇的基因型依赖性反应已有充分记录。胞质乙醛脱氢酶（ALDH - 2）在一些品系中会增加，而在另一些品系中经乙醇处理后会减少。进一步的研究表明，乙醇喂养后，ALDH - 2的mRNA（Ahd - 2 mRNA）水平会以品系依赖的方式发生改变。本报告确定了不同品系小鼠在基因组DNA水平上Ahd - 2的差异。发现了与Ahd - 2基因座相关的限制性片段长度多态性（RFLP），涉及限制性内切酶EcoRI、HindIII、Pst I和Rsa I。根据其与Ahd - 2相关的总体DNA条带模式，本研究中包含的小鼠品系可分为两组。C57BL/6J、C57BL/6J*、C57BL/10J和BALB/c品系构成第1组，而C3H/HeJ、C3H/HeSnJ、129/ReJ、Csb、SW和DBA/2J品系构成第2组。除BALB/c外，第1组代表偏好酒精的品系，而第2组是避免酒精的品系。需要进一步的研究来确定这些RFLP的生理意义（如果有的话）以及它们与乙醇偏好和回避的可能关系。
