Mougous Joseph D, Cuff Marianne E, Raunser Stefan, Shen Aimee, Zhou Min, Gifford Casey A, Goodman Andrew L, Joachimiak Grazyna, Ordoñez Claudia L, Lory Stephen, Walz Thomas, Joachimiak Andrzej, Mekalanos John J
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Science. 2006 Jun 9;312(5779):1526-30. doi: 10.1126/science.1128393.
Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter. Regulatory patterns of HSI-I suggested that the apparatus functions during chronic infections. We detected Hcp1 in pulmonary secretions of cystic fibrosis (CF) patients and Hcp1-specific antibodies in their sera. Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I-related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions.
细菌病原体经常利用蛋白质分泌来介导与宿主的相互作用。我们在此发现，铜绿假单胞菌的一个毒力位点（HSI-I）编码一种蛋白质分泌装置。该装置在离散的亚细胞位置组装，并输出Hcp1，一种形成内径为40埃环的六聚体蛋白。HSI-I的调控模式表明该装置在慢性感染期间发挥作用。我们在囊性纤维化（CF）患者的肺部分泌物中检测到Hcp1，并在他们的血清中检测到Hcp1特异性抗体。因此，HSI-I可能在CF患者铜绿假单胞菌的发病机制中起作用。与HSI-I相关的位点广泛分布于细菌病原体中，可能在介导宿主相互作用中发挥普遍作用。
