Zwick M G, Wiggs M, Paule M R
Department of Biochemistry, Colorado State University, Fort Collins 80523.
Gene. 1991 May 15;101(1):153-7. doi: 10.1016/0378-1119(91)90239-8.
A 5S RNA genomic clone has been isolated from Acanthamoeba castellanii and the sequence of the coding region plus flanking DNA was determined. This clone encodes an RNA whose sequence matches that of 5S RNA from this organism. There is sequence similarity in the 5'-flanking region to other eukaryotic 5S RNA genes which require or are greatly affected by upstream regions for transcriptional activity. The immediate 3'-flanking region has a termination sequence similar to that found in all genes that are transcribed by RNA polymerase III. The 5S RNA genes of A. castellanii are dispersed, which is highly unusual, since the majority of eukaryotic organisms contain 5S genes clustered in tandem repeats. There may be up to 480 genes encoding 5S RNA in each A. castellanii cell.
已从卡氏棘阿米巴中分离出一个5S RNA基因组克隆，并测定了编码区及其侧翼DNA的序列。该克隆编码一种RNA，其序列与来自该生物体的5S RNA序列匹配。5'侧翼区域与其他真核生物5S RNA基因存在序列相似性，这些基因的转录活性需要上游区域或受其极大影响。紧邻的3'侧翼区域有一个终止序列，类似于所有由RNA聚合酶III转录的基因中发现的终止序列。卡氏棘阿米巴的5S RNA基因是分散的，这非常不寻常，因为大多数真核生物的5S基因以串联重复的形式成簇存在。每个卡氏棘阿米巴细胞中可能有多达480个编码5S RNA的基因。
