拟南芥5S核糖体RNA编码基因的序列与组织

Sequence and organization of 5S ribosomal RNA-encoding genes of Arabidopsis thaliana.

作者信息

Campell B R, Song Y, Posch T E, Cullis C A, Town C D

机构信息

Biology Department, Case Western Reserve University, Cleveland, OH 44106.

出版信息

Gene. 1992 Mar 15;112(2):225-8. doi: 10.1016/0378-1119(92)90380-8.

DOI:10.1016/0378-1119(92)90380-8

PMID:1348233

Abstract

We have isolated a genomic clone containing Arabidopsis thaliana 5S ribosomal RNA (rRNA)-encoding genes (rDNA) by screening an A. thaliana library with a 5S rDNA probe from flax. The clone isolated contains seven repeat units of 497 bp, plus 11 kb of flanking genomic sequence at one border. Sequencing of individual subcloned repeat units shows that the sequence of the 5S rRNA coding region is very similar to that reported for other flowering plants. Four A. thaliana ecotypes were found to contain approx. 1000 copies of 5S rDNA per haploid genome. Southern-blot analysis of genomic DNA indicates that 5S rDNA occurs in long tandem arrays, and shows the presence of numerous restriction-site polymorphisms among the six ecotypes studied.

摘要

我们通过用来自亚麻的5S rDNA探针筛选拟南芥文库，分离出了一个包含拟南芥5S核糖体RNA（rRNA）编码基因（rDNA）的基因组克隆。分离出的克隆包含7个497 bp的重复单元，以及一侧边界处11 kb的侧翼基因组序列。对单个亚克隆重复单元的测序表明，5S rRNA编码区的序列与其他开花植物报道的序列非常相似。发现4种拟南芥生态型每个单倍体基因组中约含有1000个5S rDNA拷贝。基因组DNA的Southern杂交分析表明，5S rDNA以长串联阵列形式存在，并且在所研究的6种生态型中显示出许多限制性酶切位点多态性。