Zhu Xiaolong, Eibl Oliver, Scheideler Lutz, Geis-Gerstorfer Jürgen
Section of Medical Materials and Technology, Department of Prosthodontics and Medical Materials, University of Tuebingen, Germany.
J Biomed Mater Res A. 2006 Oct;79(1):114-27. doi: 10.1002/jbm.a.30706.
Osseointegration at the bone-implant interface is a prerequisite for endosseous implants to succeed in achieving and maintaining their long-term stability in bone tissue. The achievement of osseointegration is significantly affected by surface nature of implants. To optimize osseointegration, this study presents the characterization of synthesized nanocrystalline hydroxyapatite (nano HA) and in vitro studies on nano HA, nano-HA/collagen, and titanium surfaces. Voids were found within the grain of nano HA, which consisted of the shell and the core. The finding assists the clarification of microstructures of nano HA. By low-temperature mixing nano-HA sol with collagen gel (nano-HA/collagen 80:20), nano HA, and nano-HA/collagen coated on pure titanium or porous anodic titanium oxides resulted in higher wettability and lower roughness. The in vitro studies showed that porous structures produced by anodic oxides on titanium served as positive anchorage sites for cell filopodia to connect, and nano HA decreased cell attachment of osteoblasts and induced well-developed long filopodia and broad lamellipodia, thereby enhancing cellular motility. Collagen involvement enhanced cell adhesion to nano HA. Cell reactions to nano HA, nano-HA/collagen, native, and porous titanium surfaces provide some guidance for an optimal osseointegration by their application in surface modifications for implants.
骨-种植体界面的骨结合是骨内种植体在骨组织中成功实现并维持其长期稳定性的前提条件。骨结合的实现受到种植体表面性质的显著影响。为了优化骨结合，本研究展示了合成纳米晶羟基磷灰石（纳米HA）的特性以及对纳米HA、纳米HA/胶原蛋白和钛表面的体外研究。在由壳层和核心组成的纳米HA晶粒内发现了孔隙。这一发现有助于阐明纳米HA的微观结构。通过将纳米HA溶胶与胶原蛋白凝胶（纳米HA/胶原蛋白80:20）低温混合，涂覆在纯钛或多孔阳极氧化钛上的纳米HA和纳米HA/胶原蛋白具有更高的润湿性和更低的粗糙度。体外研究表明，钛上阳极氧化物产生的多孔结构作为细胞丝状伪足连接的正向锚定位点，纳米HA减少了成骨细胞的细胞附着，并诱导形成发育良好的长丝状伪足和宽片状伪足，从而增强细胞运动性。胶原蛋白的参与增强了细胞对纳米HA的黏附。细胞对纳米HA、纳米HA/胶原蛋白、天然钛和多孔钛表面的反应，通过其在种植体表面改性中的应用，为优化骨结合提供了一些指导。
