Bagnara G P, Zauli G, Re M C, Furlini G, Giovannini M, Ranieri S, Brizzi M F, La Placa M
Institute of Histology and General Embryology, University of Bologna, Italy.
Int J Cell Cloning. 1991 May;9(3):239-50. doi: 10.1002/stem.5530090308.
The production of granulocyte/macrophage colony-stimulating factor (GM-CSF) by peripheral blood (PB) light density mononuclear cells (LD-MNC), CD2+ T lymphocytes and purified CD4+ T lymphocytes was investigated in 20 human immunodeficiency virus type 1 (HIV-1) seropositive (WRII-III) individuals in comparison with 18 normal controls. GM-CSF in supernatants of stimulated cultures was determined by biological and immunoenzymatic assays. GM-CSF production by LD-MNC, CD2+ T lymphocytes and purified CD4+ T lymphocytes was significantly (p less than 0.01) reduced in HIV-1 infected individuals, especially in patients at the more advanced stages of the disease. Moreover, the number of circulating granulocyte/macrophage colony-forming units (CFU-gm) was significantly (p less than 0.01) reduced in HIV-1 seropositive subjects (31.5 +/- 4.4) compared with normal controls (78 +/- 10). There was a positive correlation (r = 0.720, p less than 0.01) between CFU-gm and GM-CSF production by LD-MNC in HIV-1 seropositive individuals. On the other hand, the absolute number of CD4+ lymphocytes did not correlate with GM-CSF production by LD-MNC (r = 0.158) or CD2+ T lymphocytes (r = 0.225). These data indicate that the impaired production of GM-CSF in HIV-1-infected individuals is not only due to a reduction in CD4+ T lymphocytes, but also to a qualitative impairment of these cells which may contribute to the loss of circulating hematopoietic progenitors in HIV-1-infected subjects.
在20名1型人类免疫缺陷病毒（HIV-1）血清阳性（WRII-III）个体中，研究外周血（PB）轻密度单核细胞（LD-MNC）、CD2⁺ T淋巴细胞和纯化的CD4⁺ T淋巴细胞产生粒细胞/巨噬细胞集落刺激因子（GM-CSF）的情况，并与18名正常对照者进行比较。通过生物学和免疫酶测定法测定刺激培养上清液中的GM-CSF。在HIV-1感染个体中，尤其是疾病晚期患者，LD-MNC、CD2⁺ T淋巴细胞和纯化的CD4⁺ T淋巴细胞产生GM-CSF的能力显著降低（p<0.01）。此外，与正常对照者（78±10）相比，HIV-1血清阳性受试者的循环粒细胞/巨噬细胞集落形成单位（CFU-gm）数量显著减少（p<0.01）（31.5±4.4）。在HIV-1血清阳性个体中，CFU-gm与LD-MNC产生GM-CSF之间存在正相关（r = 0.720，p<0.01）。另一方面，CD4⁺淋巴细胞的绝对数量与LD-MNC（r = 0.158）或CD2⁺ T淋巴细胞（r = 0.225）产生GM-CSF无关。这些数据表明，HIV-1感染个体中GM-CSF产生受损不仅是由于CD4⁺ T淋巴细胞减少，还由于这些细胞的质量受损，这可能导致HIV-1感染受试者循环造血祖细胞的丢失。
