Addas-Carvalho M, Salles T S I, Saad S T O
Hematology and Transfusion Medicine Center, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Transfus Med. 2006 Jun;16(3):184-91. doi: 10.1111/j.1365-3148.2006.00665.x.
Cytokines are associated with inflammatory responses including febrile non-hemolytic transfusion reactions (FNHTR). Moreover, there are some polymorphisms of these cytokine genes associated with different levels of gene expression. The aim of the present study was to investigate the association of inflammatory cytokine gene polymorphisms with the occurrence of FNHTR in multitransfused patients. We studied two groups of transfused patients: one presenting FNHTR before 20 transfusions of red blood cells concentrates and the other which never presented FNHTR even after 20 transfusions. The gene polymorphisms studied were IL1B-511C/T and +3953C/T, IL1RN (intron 2, variable number tandem repeat), IL6-174G/C, IL10-1082G/A and -819C/T, TNF-308G/A and LTA+253G/A using polymerase chain reaction and restriction digestion or sequencing methods. An association of IL1RN*2.2 genotype with the occurrence of precocious FNHTR (P < 0.025) was detected. This allele and this genotype have been related with higher serum levels of interleukin (IL)-1beta in vivo and higher promoter activity. No other association was demonstrated. The association of gene polymorphisms related with the increase of inflammatory cytokine gene expression may be a relevant factor in FNHTR and requires confirmation.
细胞因子与包括发热性非溶血性输血反应（FNHTR）在内的炎症反应相关。此外，这些细胞因子基因存在一些多态性，与不同水平的基因表达相关。本研究的目的是调查炎症细胞因子基因多态性与多次输血患者发生FNHTR之间的关联。我们研究了两组输血患者：一组在输注20次红细胞浓缩液之前出现FNHTR，另一组即使在输注20次后也从未出现FNHTR。使用聚合酶链反应和限制性消化或测序方法研究的基因多态性包括IL1B - 511C/T和 + 3953C/T、IL1RN（内含子2，可变数目串联重复序列）、IL6 - 174G/C、IL10 - 1082G/A和 - 819C/T、TNF - 308G/A以及LTA + 253G/A。检测到IL1RN*2.2基因型与早熟FNHTR的发生相关（P < 0.025）。该等位基因和该基因型在体内与较高水平的白细胞介素（IL）-1β血清水平以及较高的启动子活性相关。未发现其他关联。与炎症细胞因子基因表达增加相关的基因多态性关联可能是FNHTR中的一个相关因素，需要进一步证实。
