Addas-Carvalho M, Origa A F, Saad S T O
Hematology and Transfusion Medicine Center, Universidade Estadual de Campinas-UNICAMP, Brazil.
Transfusion. 2004 Jul;44(7):996-1003. doi: 10.1111/j.1537-2995.2004.03257.x.
Cytokines (IL-1beta and TNF) generated by WBCs during storage of PLT concentrates have been associated with febrile nonhemolytic transfusion reactions.
This study was undertaken to investigate whether there is an association between the polymorphisms of IL1B -511C/T and +3953C/T, IL1RN intron 2 VNTR and TNFA-308G/A genes and the increase of cytokines during the storage of PLT concentrates produced by plasma-rich PLTS (PRP-PC) or apheresis PLTs.
Thirty PRP-PCs were studied and a progressive increase of IL-1beta and TNF during storage was revealed. IL1-beta and TNF levels were inversely correlated with the content of PLTs in PRP-PCs detected on Day 3 (p = 0.004) and Day 5 (p = 0.019), but not on Day 7. There was association of IL1B-511T polymorphism and IL-1beta levels (Day 5, p = 0.063, only tendency and on Day 7, p = 0.038, significant). There was no association of the other polymorphisms (IL1B+3953C/T, IL1RN intron 2 and TNFA-308G/A) with their respective cytokines.
The great variation of cytokine levels in the plasma of PLT concentrates (PCs) during storage may also be caused by cytokine gene polymorphisms, as well as WBC contamination, material that the bags are made of, and storage time, as previously described.
血小板浓缩物储存期间白细胞产生的细胞因子（IL-1β和TNF）与发热性非溶血性输血反应有关。
本研究旨在调查IL1B -511C/T和+3953C/T、IL1RN内含子2 VNTR以及TNFA -308G/A基因的多态性与富含血浆血小板（PRP-PC）或单采血小板储存期间血小板浓缩物中细胞因子增加之间是否存在关联。
研究了30份PRP-PC，发现储存期间IL-1β和TNF呈逐渐增加。IL-1β和TNF水平与第3天（p = 0.004）和第5天（p = 0.019）检测到的PRP-PC中血小板含量呈负相关，但与第7天无关。IL1B-511T多态性与IL-1β水平有关联（第5天，p = 0.063，仅为趋势，第7天，p = 0.038，显著）。其他多态性（IL1B+3953C/T、IL1RN内含子2和TNFA -308G/A）与其各自的细胞因子无关联。
血小板浓缩物（PC）血浆中细胞因子水平在储存期间的巨大变化可能也由细胞因子基因多态性引起，以及如先前所述的白细胞污染、袋子制作材料和储存时间。
