Bernardos Rebecca L, Raymond Pamela A
Neuroscience Graduate Program, 4402 Kresge III, University of Michigan, Ann Arbor, MI 48109-0520, USA.
Gene Expr Patterns. 2006 Oct;6(8):1007-13. doi: 10.1016/j.modgep.2006.04.006. Epub 2006 Jun 9.
We have generated transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. Transgenic lines Tg(gfap:GFP) were generated from three founders; the results presented here are from the mi2001 line. GFP expression was first visible in the living embryo at the tail bud-stage, then in the developing brain by the 5-somite-stage ( approximately 12 h post-fertilization, hpf) and then spreading posteriorly along the developing spinal cord by the 12-somite stage (approximately 15 hpf). At 24 hpf GFP-expressing cells were in the retina and lens. By 72 hpf GFP expression levels were strong and localized to the glia of the brain, neural retina, spinal cord, and ventral spinal nerves, with moderate expression in the enteric nervous system and weaker levels in the olfactory sensory placode and otic capsule. GFP expression in glia co-localized with anti-GFAP antibodies, but did not co-localize with the neuronal antibodies HuC/D or calretinin in mature neurons.
我们已经培育出了转基因斑马鱼，这些斑马鱼在斑马鱼胶质纤维酸性蛋白（GFAP）调控元件的驱动下，在神经胶质细胞中表达绿色荧光蛋白（GFP）。转基因品系Tg(gfap:GFP)由三位奠基鱼培育而成；此处展示的结果来自mi2001品系。GFP表达最初在尾芽期的活体胚胎中可见，然后在5体节期（受精后约12小时，hpf）的发育中的大脑中可见，接着在12体节期（约15 hpf）沿发育中的脊髓向后扩散。在24 hpf时，表达GFP的细胞位于视网膜和晶状体中。到72 hpf时，GFP表达水平很强，且定位于大脑、神经视网膜、脊髓和腹侧脊神经的神经胶质细胞，在肠神经系统中有中度表达，在嗅觉感觉基板和耳囊中表达较弱。神经胶质细胞中的GFP表达与抗GFAP抗体共定位，但在成熟神经元中不与神经元抗体HuC/D或钙视网膜蛋白共定位。
