Sen Selda, Doger Firuzan Kacan, Sen Serdar, Aydin Osman N, Karul Aslihan, Dost Turhan
Adnan Menderes University, Medical Faculty, Anesthesiology and Reanimation Department, Turkey.
Eur J Pharmacol. 2006 Jul 17;541(3):191-7. doi: 10.1016/j.ejphar.2006.05.022. Epub 2006 May 22.
The aim of this study is firstly, to determine the preventive effect of chronic usage of combination of nitroglycerin and lornoxicam on gastrointestinal and renal side effects and secondly, to investigate the oxidative and antioxidative effects of this combination in rats.
Thirty-seven Wistar male rats were divided into five groups for 15 days; isotonic group (n = 8, sodium chloride 0.09, Group ISO), lornoxicam group (n = 8, lornoxicam 1.3 mg/kg, Group L), nitroglycerin group (n = 6, nitroglycerin 1 mg/kg, Group NTG), lornoxicam-nitroglycerin combination group (n = 8, 1.3 mg/kg lornoxicam + 1 mg/kg nitroglycerin, Group L-NTG), and control group (n = 7, no drug was administered, Group C). Nitric oxide, malondialdehyde, reduced glutathione (GSH), catalase, interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha concentrations were measured before drug injection and on fifteenth day in all blood samples. Gastrointestinal and renal biopsies were performed on fifteenth day.
Two rats died on tenth and twelfth days in Group L. There were significant differences in Group L compared to the other groups for the lesions of stomach and kidney (p = 0.01, p = 0.028 respectively). Gastric ulceration was occurred in a rat in Group L. Malondialdehyde, TNF-alpha, and IL-6 levels decreased in NTG and L-NTG groups, whereas catalase and glutathion levels increased in NTG, L and L-NTG groups compared to control group (p < 0.05).
Lornoxicam may cause gastrointestinal and renal side effects without oxidative stress. Adding nitroglycerin to lornoxicam for chronic treatment may prevent these side effects and enhance antioxidative effect compared to the use of lornoxicam alone in rats.
本研究的目的首先是确定长期联合使用硝酸甘油和氯诺昔康对胃肠道和肾脏副作用的预防作用，其次是研究该组合在大鼠体内的氧化和抗氧化作用。
将37只雄性Wistar大鼠分为五组，为期15天；等渗组（n = 8，0.09%氯化钠，ISO组）、氯诺昔康组（n = 8，氯诺昔康1.3 mg/kg，L组）、硝酸甘油组（n = 6，硝酸甘油1 mg/kg，NTG组）、氯诺昔康-硝酸甘油联合组（n = 8，1.3 mg/kg氯诺昔康 + 1 mg/kg硝酸甘油，L-NTG组）和对照组（n = 7，未给药，C组）。在所有血样中，于注射药物前和第15天测量一氧化氮、丙二醛、还原型谷胱甘肽（GSH）、过氧化氢酶、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α浓度。在第15天进行胃肠道和肾脏活检。
L组有两只大鼠分别在第10天和第12天死亡。L组与其他组相比，胃和肾脏病变存在显著差异（分别为p = 0.01，p = 0.
