Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 32003, Taiwan.
J Hazard Mater. 2010 Jun 15;178(1-3):706-12. doi: 10.1016/j.jhazmat.2010.01.145. Epub 2010 Feb 6.
A combined solvent extraction and biphasic biodegradation process was proposed to remove organic pollutants from highly saline solutions, where the organic solvent is biocompatible and the organic-aqueous interface remains quasi-quiescent during the process. Phenol and Pseudomonas putida BCRC 14365 were selected as the model organic compound and biomass, respectively. The effects of added NaCl concentration (50-200 g/L) and pH (1.0-9.0) in synthetic solutions on phenol removal and cell growth were studied at 30 degrees C. The initial cell concentration was fixed at 0.025 g/L. Within the examined range, the adjustment of solution pH to 3.0 resulted in the best removal performance of 1100 mg/L phenol from such saline solutions (an apparent removal rate of about 20 mg/(Lh)) when using kerosene as the organic solvent. The overall process appeared to be favored when the salt concentration in saline solution was in the range of 100-150 g/L. The application potentials of such a triphasic process for the removal of toxic organics (phenol) from highly saline and acidic wastewaters were finally demonstrated in fed-batch mode.
提出了一种联合溶剂萃取和双相反生物降解工艺，用于从高盐溶液中去除有机污染物，其中有机溶剂具有生物相容性，并且在整个过程中有机-水界面保持近乎静止。选择苯酚和恶臭假单胞菌 BCRC 14365 分别作为模型有机化合物和生物质。在 30℃下，研究了添加 NaCl 浓度（50-200 g/L）和 pH 值（1.0-9.0）对合成溶液中苯酚去除和细胞生长的影响。初始细胞浓度固定为 0.025 g/L。在所考察的范围内，当使用煤油作为有机溶剂时，将溶液 pH 值调节至 3.0 可使高盐溶液中 1100mg/L 苯酚的去除效果最佳（表观去除速率约为 20mg/（Lh））。当盐浓度在 100-150g/L 范围内时，整个过程似乎更有利。最后，在分批进料模式下，证明了这种三相工艺用于去除高盐和酸性废水中有毒有机物（苯酚）的应用潜力。
