利用管家基因gdh（zwf）和gki进行多位点序列分型，以区分肺炎链球菌与缓症链球菌和口腔链球菌。
Use of the housekeeping genes, gdh (zwf) and gki, in multilocus sequence typing to differentiate Streptococcus pneumoniae from Streptococcus mitis and Streptococcus oralis.
作者信息
Ip Margaret, Chi Fang, Chau Shirley S L, Hui Mamie, Tang Julian, Chan Paul K S
机构信息
Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
出版信息
Diagn Microbiol Infect Dis. 2006 Nov;56(3):321-4. doi: 10.1016/j.diagmicrobio.2006.04.013. Epub 2006 Jun 12.
Abstract
Polymerase chain reaction and sequencing of the housekeeping genes, gdh (zwf) and gki, based on the primers and alleles from multilocus sequence typing can be used to delineate and support the identity of clinical isolates of Streptococcus pneumoniae and differentiate from the closely related Streptococcus mitis and Streptococcus oralis.
摘要
基于多位点序列分型的引物和等位基因，对管家基因gdh（zwf）和gki进行聚合酶链反应和测序，可用于确定和支持肺炎链球菌临床分离株的身份，并与密切相关的缓症链球菌和口腔链球菌相区分。
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