Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
J Clin Microbiol. 2013 Mar;51(3):835-40. doi: 10.1128/JCM.02920-12. Epub 2012 Dec 26.
A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently detected with size differences, whereas no PCR products were amplified from any of the reference strains tested. A pilot study of 47 human oral swab specimens was conducted in parallel, and the mPCR assay identified S. pneumoniae in 1 sample, S. mitis in 8 samples, and S. oralis in 2 samples, providing a powerful means for characterization at the level of species compared with traditional culture analysis. Our results suggest that the mPCR protocol presented here is a sensitive and promising tool for the rapid detection and discrimination of S. pneumoniae, S. mitis, and S. oralis from clinical specimens.
建立了一种多重 PCR（mPCR）方法，用于同时检测肺炎链球菌、缓症链球菌和口腔链球菌中的 gyrB 基因，并用 141 株球菌菌株评估了其特异性。从肺炎链球菌、缓症链球菌和口腔链球菌菌株中提取的基因组 DNA 经大小差异可有效检测，而从任何测试的参考菌株中均未扩增出 PCR 产物。对 47 个人口腔拭子标本进行了平行的初步研究，mPCR 检测法在 1 个样本中鉴定出肺炎链球菌，在 8 个样本中鉴定出缓症链球菌，在 2 个样本中鉴定出口腔链球菌，与传统的培养分析相比，这为种水平的特征提供了有力的手段。我们的结果表明，本文提出的 mPCR 方案是一种快速、灵敏和有前途的工具，可用于从临床标本中快速检测和区分肺炎链球菌、缓症链球菌和口腔链球菌。
