一种功能化钌（II）混合多吡啶配合物[Ru(bpy)₂ppd]²⁺的DNA相互作用
DNA interactions of a functionalized ruthenium(II) mixed-polypyridyl complex [Ru(bpy)2ppd]2+.
作者信息
Gao Feng, Chao Hui, Zhou Feng, Yuan Yi-Xian, Peng Bin, Ji Liang-Nian
机构信息
Department of Chemistry, State Key Laboratory of Optoelectronic Materials and Technologies, The Key Laboratory of Gene Enginerring of Ministry of Education, Sun Yat-Sen University, Guanghou 510275, PR China.
出版信息
J Inorg Biochem. 2006 Sep;100(9):1487-94. doi: 10.1016/j.jinorgbio.2006.04.008.
A novel polypyridyl ligand pteridino[7,6-f][1,10]phenanthroline-1,13(10H,12H)-dione (ppd) and its ruthenium(II) complex [Ru(bpy)2ppd]2+ have been synthesized and characterized by elemental analysis, electrospray mass spectra and 1H NMR. The interaction of the complex with calf thymus DNA was investigated by spectroscopic and viscosity measurements. The results suggest that the complex binds to DNA via an intercalative mode and serves as a molecular "light switch" for DNA. Moreover, the complex has been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm. The mechanism studies reveal that singlet oxygen (1O2) and superoxide anion radical (O2*(-))play a significant role in the photocleavage.
一种新型的多吡啶配体蝶啶并[7,6-f][1,10]菲咯啉-1,13(10H,12H)-二酮(ppd)及其钌(II)配合物[Ru(bpy)2ppd]2+已通过元素分析、电喷雾质谱和1H NMR进行了合成和表征。通过光谱和粘度测量研究了该配合物与小牛胸腺DNA的相互作用。结果表明，该配合物通过插入模式与DNA结合，并作为DNA的分子“光开关”。此外，已发现该配合物在365nm照射下能促进质粒DNA pBR322的光裂解。机理研究表明，单线态氧(1O2)和超氧阴离子自由基(O2*(-))在光裂解中起重要作用。
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