Wu Yunhua, Hu Shengshui
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Bioelectrochemistry. 2007 May;70(2):335-41. doi: 10.1016/j.bioelechem.2006.05.001. Epub 2006 May 6.
Colloid Au (Au(nano)) with a diameter of about 10 nm was prepared and used in combination with dihexadecylphosphate (DHP) to immobilize glucose oxidase (GOD) onto the surface of a graphite electrode (GE). The direct electrochemistry of GOD confined in the composite film was investigated. The immobilized GOD displayed a pair of redox peaks with a formal potential of -0.475 mV in pH 7.0 O(2)-free phosphate buffers at scan rate of 150 mV s(-1). The GOD in the composite film retained its bioactivity and could catalyze the reduction of dissolved oxygen. Upon the addition of glucose, the reduction peak current of dissolved oxygen decreased, which could be developed for glucose determination. A calibration linear range of glucose was 0.5-9.3 mM with a detection limit of 0.1 mM and a sensitivity of 1.14 microA mM(-1). The glucose biosensor showed good reproducibility and stability. The general interferences that coexisted in human serum sample such as ascorbic acid and uric acid did not affect glucose determination.
制备了直径约为10 nm的胶体金（Au（纳米）），并将其与磷酸二己酯（DHP）结合使用，将葡萄糖氧化酶（GOD）固定在石墨电极（GE）表面。研究了复合膜中GOD的直接电化学。在pH 7.0无氧磷酸盐缓冲液中，扫描速率为150 mV s(-1)时，固定化的GOD显示出一对形式电位为-0.475 mV的氧化还原峰。复合膜中的GOD保留了其生物活性，并且可以催化溶解氧的还原。加入葡萄糖后，溶解氧的还原峰电流降低，可用于葡萄糖测定。葡萄糖的校准线性范围为0.5-9.3 mM，检测限为0.1 mM，灵敏度为1.14 μA mM(-1)。该葡萄糖生物传感器具有良好的重现性和稳定性。人血清样品中同时存在的一般干扰物，如抗坏血酸和尿酸，不影响葡萄糖测定。
