Wang Xueying, Gu Haifang, Yin Fan, Tu Yifeng
Institute of Analytical Chemistry, Department of Chemistry, Dushu-Lake Campus, Suzhou University, Suzhou 215123, PR China.
Biosens Bioelectron. 2009 Jan 1;24(5):1527-30. doi: 10.1016/j.bios.2008.09.025. Epub 2008 Oct 14.
Based on electrodeposition of Prussian blue (PB) and chitosan (CS) directly on gold electrode, a hybrid film of PB/CS has been prepared. PB in this film shows a good stability compared with pure PB film when it worked in neutral and weak alkalescent solution and can act as redox mediator. It provides the potential application of such film in biosensor fabrication. A glucose biosensor was fabricated by electrodepositing glucose oxidase (GOD)/CS film on this PB/CS modified electrode. The optimum experimental conditions of biosensor for the detection of glucose have been studied in detail. Under the optimal conditions, a linear dependence of the catalytic current upon glucose concentration was obtained in the range of 2x10(-6) to 4x10(-4)M with a detection limit of 3.97x10(-7)M. The resulting biosensor could be applied to detect the blood sugar in real samples without any pretreatment.
基于普鲁士蓝（PB）和壳聚糖（CS）直接电沉积在金电极上，制备了PB/CS复合膜。当该复合膜在中性和弱碱性溶液中工作时，其中的PB与纯PB膜相比表现出良好的稳定性，并且可以作为氧化还原介质。这为该复合膜在生物传感器制造中的潜在应用提供了可能。通过在该PB/CS修饰电极上电沉积葡萄糖氧化酶（GOD）/CS膜制备了葡萄糖生物传感器。详细研究了该生物传感器检测葡萄糖的最佳实验条件。在最佳条件下，催化电流与葡萄糖浓度在2×10⁻⁶至4×10⁻⁴M范围内呈线性关系，检测限为3.97×10⁻⁷M。所得生物传感器可用于直接检测实际样品中的血糖，无需任何预处理。
