Burgess D J, Kwok K K, Megremis P T
Department of Pharmaceutics, College of Pharmacy, University of Illinois, Chicago 60612.
J Pharm Pharmacol. 1991 Apr;43(4):232-6. doi: 10.1111/j.2042-7158.1991.tb06674.x.
Complex coacervation between oppositely charged albumin and acacia mixtures has been studied, and the applicability of the various theoretical treatments of complex coacervation (the Voorn-Overbeek, Veis-Aranyi, Nakajima-Sato, and Tainaka theories) to this system has been assessed. Under optimum conditions where maximum coacervate yield occurred, the Voorn-Overbeek theory appeared to apply. However, away from the optimum coacervation conditions, coacervate sol formation was observed, which is in accordance with the Veis-Aranyi and Tainaka theories. Microelectrophoretic measurements were used to determine optimum pH and ionic strength conditions for maximum coacervation, based on the method of Burgess & Carless (1984). The effects of pH and ionic strength on coacervate yield are reported. Around the optimum conditions for maximum coacervation a viscous coacervate phase and a relatively clear equilibrium phase are formed.
已对带相反电荷的白蛋白和阿拉伯胶混合物之间的复合凝聚进行了研究，并评估了复合凝聚的各种理论处理方法（沃恩 - 奥弗贝克理论、维斯 - 阿兰伊理论、中岛 - 佐藤理论和田中理论）对该体系的适用性。在凝聚物产量最高的最佳条件下，沃恩 - 奥弗贝克理论似乎适用。然而，在远离最佳凝聚条件时，观察到了凝聚溶胶的形成，这与维斯 - 阿兰伊理论和田中理论一致。基于伯吉斯和卡莱斯（1984年）的方法，使用微电泳测量来确定最大凝聚的最佳pH值和离子强度条件。报告了pH值和离子强度对凝聚物产量的影响。在最大凝聚的最佳条件附近，形成了粘性凝聚相和相对澄清的平衡相。
