Wang Fang, Huo Sheng Nan, Guo Jing, Zhang Xian Sheng
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China.
Planta. 2006 Oct;224(5):1129-40. doi: 10.1007/s00425-006-0294-0. Epub 2006 May 16.
The D-type cyclin genes play key roles in plant development of Arabidopsis. To investigate the functions of cyclins in monocots, a putative ortholog of cyclin D2 genes was isolated from wheat shoot tissues, and designated as Triae;CYCD2;1. The transcripts of Triae;CYCD2;1 were primarily localized in the proliferating tissues, particularly, in shoot apical meristem and leaf primordia of wheat plants. Ectopic expression of Triae;CYCD2;1 in Arabidopsis affected plant morphology and retarded plant growth. Further examination showed that the promotion of cell division and the inhibition of cell differentiation occurred in both transgenic plants and tissues. In vitro experiments indicated that Triae;CYCD2;1 had functional roles in responding to cytokinin and auxin. Molecular analysis revealed that the transcript levels of several cell cycle-associated genes, particularly Arath;CYCD3;1, were increased in the Arabidopsis plants with the expressing Triae;CYCD2;1. The results in this study provide new information on D-type cyclin in wheat.
D型细胞周期蛋白基因在拟南芥的植物发育中起关键作用。为了研究细胞周期蛋白在单子叶植物中的功能，从小麦茎组织中分离出一个假定的细胞周期蛋白D2基因直系同源物，并将其命名为Triae;CYCD2;1。Triae;CYCD2;1的转录本主要定位在增殖组织中，特别是在小麦植株的茎尖分生组织和叶原基中。Triae;CYCD2;1在拟南芥中的异位表达影响了植物形态并延缓了植物生长。进一步检查表明，转基因植物和组织中都出现了细胞分裂的促进和细胞分化的抑制。体外实验表明，Triae;CYCD2;1在响应细胞分裂素和生长素方面具有功能作用。分子分析显示，在表达Triae;CYCD2;1的拟南芥植物中，几个与细胞周期相关的基因，特别是Arath;CYCD3;1的转录水平有所增加。本研究结果为小麦中的D型细胞周期蛋白提供了新信息。
