Lera S, Pellegrini D
ICRAM, Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy.
Environ Monit Assess. 2006 Aug;119(1-3):1-13. doi: 10.1007/s10661-005-9000-0. Epub 2006 Jun 10.
Several experiments were performed to evaluate the fertilization capability of Paracentrotus lividus sea urchin gametes, which are usually used in fertilization tests, but which, until now, were employed only for few hours after spawning. The aim of this work is to evaluate the possibility of performing the bioassay for longer periods, employing 'conserved' gametes. As far as the method, the sperm was stored 'dry' at 4 degrees C; eggs were conserved in an antibiotic solution at 4 degrees C, and they were exposed both to copper solutions as reference toxicant and to aqueous matrices. The use of conserved gametes in the fertilization bioassay lead to EC50 values which were acceptable for about 3 days. Moreover, these experiments permitted to identify the limiting factor: the male gamete.
进行了多项实验以评估紫球海胆配子的受精能力，紫球海胆配子通常用于受精测试，但迄今为止，产卵后仅使用数小时。这项工作的目的是评估使用“保存”的配子进行更长时间生物测定的可能性。就方法而言，精子在4℃下“干燥”保存；卵子保存在4℃的抗生素溶液中，并使其暴露于作为参考毒物的铜溶液和水相中。在受精生物测定中使用保存的配子可得到约3天内可接受的半数有效浓度（EC50）值。此外，这些实验有助于确定限制因素：雄配子。
