[中国辽宁汉族人群HLA - DRB1基因位点的多态性分布]
[Polymorphism distribution of HLA-DRB1 gene locus in Han race population in Liaoning of China].
作者信息
Qu Zhe, Li Chun-mei, Li Jian-ping
机构信息
Liaoning Blood Center, Shenyang, Liaoning, 110044 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):349-51.
OBJECTIVE
To elucidate the genotype distribution of HLA-DRB1 polymorphisms in the Han race population in Liaoning of China and to evaluate its value on forensic medicine.
METHODS
The blood DNA samples from 13265 unrelated Chinese individuals of Han race population in Liaoning province were investigated and HLA-DRB1 alleles were genotyped with PCR-SSP and PCR-SSOP in low resolution level.
RESULTS
Thirteen HLA-A alleles were found, in which HLA-DRB115, 09, 12, 07 were the commonest but HLA-DRB103(18), HLA-DRB1*14(8) were absent. The population data conformed to the Hardy-Weinberg equilibrium(chi-square was 73.34, df was 78, P> 0.5). The exclusion probability of paternity was 64.7%, the power of discrimination was 97.9% and the heterozygosity was 89.4%.
CONCLUSION
The HLA-DRB1 polymorphism of Liaoning Han populations show the special characters and HLA-DRB1 genotyping is useful genetic marker to paternity testing and personal identification.
目的
阐明中国辽宁汉族人群中HLA - DRB1基因多态性的基因型分布，并评估其在法医学中的价值。
方法
对辽宁省13265名无血缘关系的汉族个体的血液DNA样本进行研究，采用聚合酶链反应 - 序列特异性引物（PCR - SSP）和聚合酶链反应 - 序列特异性寡核苷酸探针（PCR - SSOP）在低分辨率水平对HLA - DRB1等位基因进行基因分型。
结果
共发现13个HLA - A等位基因，其中HLA - DRB115、09、12、07最为常见，但未发现HLA - DRB103(18)、HLA - DRB1*14(8)。群体数据符合哈迪 - 温伯格平衡（卡方值为73.34，自由度为78，P>0.5）。父权排除概率为64.7%，鉴别能力为97.9%，杂合度为89.4%。
结论
辽宁汉族人群的HLA - DRB1基因多态性具有特殊性，HLA - DRB1基因分型是亲子鉴定和个人识别的有用遗传标记。
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