Matsumoto Yuki, Ogawa Yasutaka, Yoshida Rie, Nakata Akinori, Kasai Hiroshi, Ohba Kenichi, Ohta Hisayoshi
National Institute of Industrial Health, Kawasaki.
Nihon Eiseigaku Zasshi. 2006 May;61(3):357-65. doi: 10.1265/jjh.61.357.
In this study, we aim to examine whether the length of sleep modifies the level of urinary 8-hydroxy-2'-deoxyguanosine (8-OH-dG).
Subjects were 146 workers who were engaged in management, clerical work, laboratory work, or business at a certain company. We obtained information on the subjects concerning gender, body mass index, drinking and smoking habits, and the lengths of habitual and previous night sleep among others using self-reported questionnaires, which were then confirmed by interview. Urine specimens were collected in the morning and those from the second or later void were used to measure 8-OH-dG and creatinine levels. The amount of 8-OH-dG normalized by creatinine content was used as the indicator of 8-OH-dG excretion (Spot Urine 8-OH-dG: SU8-OH-dG). We excluded subjects who took sleeping pills the previous night or habitually, and those whose levels were beyond the three-standard-deviation range. The subjects were then classified into three equal-sized groups according to the length of sleep, either habitual or the previous night.
(1) A long or a short previous night's sleep, drinking alcohol more than once a week, and habitual use of medicine increased SU8-OH-dG level among female subjects. (2) A short previous night's sleep and habitual smoking, and a short previous night's sleep and habitual use of medicine had interactions which increased SU8-OH-dG level among male subjects but the length of a previous night's sleep itself did not have an effect on SU8-OH-dG level. (3) The length of habitual sleep had no effect on SU8-OH-dG level.
在本研究中，我们旨在探讨睡眠时长是否会改变尿8-羟基-2'-脱氧鸟苷（8-OH-dG）水平。
研究对象为某公司146名从事管理、文职、实验室工作或商务工作的员工。我们通过自填问卷获取了受试者的性别、体重指数、饮酒和吸烟习惯、习惯性睡眠时长和前一晚睡眠时长等信息，之后通过访谈进行了确认。于早晨采集尿液样本，采用第二次及以后排尿的样本测量8-OH-dG和肌酐水平。以肌酐含量标准化后的8-OH-dG量作为8-OH-dG排泄指标（即时尿8-OH-dG：SU8-OH-dG）。我们排除了前一晚或习惯性服用安眠药的受试者以及水平超出三个标准差范围的受试者。然后根据习惯性睡眠时长或前一晚睡眠时长将受试者分为三个等规模的组。
（1）前一晚睡眠过长或过短、每周饮酒超过一次以及习惯性用药会使女性受试者的SU8-OH-dG水平升高。（2）前一晚睡眠过短与习惯性吸烟、前一晚睡眠过短与习惯性用药之间存在相互作用，会使男性受试者的SU8-OH-dG水平升高，但前一晚睡眠时长本身对SU8-OH-dG水平没有影响。（3）习惯性睡眠时长对SU8-OH-dG水平没有影响。
