Wu Ming-Tsang, Pan Chih-Hong, Huang Yeou-Lih, Tsai Perng-Jy, Chen Chiou-Jong, Wu Trong-Neng
Graduate Institute of Occupational Safety and Health and Department of Occupational Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Environ Mol Mutagen. 2003;42(2):98-105. doi: 10.1002/em.10176.
Coke-oven workers (COWs) are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons (PAH). Urinary 8-hydroxy-2-deoxyguanosine (8-OH-dG) and 1-hydroxypyrene (1-OHP) are biological markers of oxidative DNA damage and PAH metabolism, respectively. We investigated the relationship between urinary 8-OH-dG and 1-OHP in 217 Taiwanese COWs, 55 topside-oven, and 162 sideoven workers. For topside-oven workers, mean 8-OH-dG and 1-OHP concentrations (ng/ml +/- SD) were 13.8 +/- 12.0 and 93.5 +/- 104.4, respectively. These levels were significantly higher than those for sideoven workers: 10.2 +/- 7.9 ng/ml (P = 0.04) and 19.8 +/- 28.6 ng/ml (P < 0.001), respectively. Individual urinary 8-OH-dG concentrations were directly correlated with urinary 1-OHP concentrations: the higher the 1-OHP level, the higher the 8-OH-dG level (Spearman correlation coefficients: r = 0.43, P < 0.0001, n = 217). Multiple regression analysis indicated that a 10-fold increase in 1-OHP was associated with a 1.91-fold increase in 8-OH-dG. Compared to no vitamin intake, intake of at least one multiple vitamin pill per week reduced 8-OH-dG excretion (P = 0.02). Our findings suggest that urinary 1-OHP and 8-OH-dG reflect occupational PAH exposure and oxidative DNA damage in COWs. In addition, multiple vitamins may reduce oxidative stress caused by PAH exposure.
焦炉工人职业性接触高浓度的多环芳烃（PAH）。尿8-羟基-2-脱氧鸟苷（8-OH-dG）和1-羟基芘（1-OHP）分别是氧化性DNA损伤和PAH代谢的生物标志物。我们调查了217名台湾焦炉工人、55名顶炉工人和162名侧炉工人尿8-OH-dG与1-OHP之间的关系。对于顶炉工人，尿8-OH-dG和1-OHP的平均浓度（ng/ml±标准差）分别为13.8±12.0和93.5±104.4。这些水平显著高于侧炉工人：分别为10.2±7.9 ng/ml（P = 0.04）和19.8±28.6 ng/ml（P < 0.001）。个体尿8-OH-dG浓度与尿1-OHP浓度直接相关：1-OHP水平越高，8-OH-dG水平越高（Spearman相关系数：r = 0.43，P < 0.0001，n = 217）。多元回归分析表明，1-OHP增加10倍与8-OH-dG增加1.91倍相关。与不摄入维生素相比，每周至少摄入一粒复合维生素片可减少8-OH-dG排泄（P = 0.02）。我们的研究结果表明，尿1-OHP和8-OH-dG反映了焦炉工人的职业性PAH暴露和氧化性DNA损伤。此外，复合维生素可能会降低PAH暴露引起的氧化应激。
