Du Jin-Zhi, Chen Dong-Ping, Wang Yu-Cai, Xiao Chun-Sheng, Lu Yi-Jie, Wang Jun, Zhang Guang-Zhao
Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
Biomacromolecules. 2006 Jun;7(6):1898-903. doi: 10.1021/bm051003c.
A novel biodegradable amphiphilic brush-coil block copolymer consisting of poly(epsilon-caprolactone) and PEGylated polyphosphoester was synthesized by ring opening polymerization. The composition and structure of the copolymer were characterized by 1H NMR, 13C NMR, and FT-IR, and the molecular weight and molecular weight distribution were analyzed by gel permeation chromatograph (GPC) measurements to confirm the diblock structure. These amphiphilic copolymers formed micellar structures in water, and the critical micelle concentrations (CMCs) were around 10(-3) mg/mL, which was determined using pyrene as a fluorescence probe. Transmission electron microscopy (TEM) images showed that the micelles took an approximately spherical shape with core-shell structure, which was further demonstrated by laser light scattering (LLS) technique. The degradation behavior of the polymeric micelle was also investigated in the presence of Pseudomonas lipase and characterized by GPC measurement. Such polymer micelles from brush-coil block copolymers are expected to have wide utility in the field of drug delivery.
通过开环聚合反应合成了一种新型的可生物降解的两亲性刷-线圈型嵌段共聚物，该共聚物由聚（ε-己内酯）和聚乙二醇化聚磷酸酯组成。通过1H NMR、13C NMR和FT-IR对共聚物的组成和结构进行了表征，通过凝胶渗透色谱（GPC）测量分析了其分子量和分子量分布，以确认其两嵌段结构。这些两亲性共聚物在水中形成胶束结构，以芘作为荧光探针测定其临界胶束浓度（CMC）约为10（-3）mg/mL。透射电子显微镜（TEM）图像显示胶束呈近似球形的核壳结构，激光光散射（LLS）技术进一步证实了这一点。还研究了在假单胞菌脂肪酶存在下聚合物胶束的降解行为，并通过GPC测量进行了表征。这种由刷-线圈型嵌段共聚物形成的聚合物胶束有望在药物递送领域具有广泛的应用。
