Schoeler Bjoern, Delorme Nicolas, Doench Ingo, Sukhorukov Gleb B, Fery Andreas, Glinel Karine
UMR 6522, Polymères, Biopolymères, Membranes, CNRS - Université de Rouen, Bd Maurice de Broglie, F-76821 Mont-Saint-Aignan, France.
Biomacromolecules. 2006 Jun;7(6):2065-71. doi: 10.1021/bm060378a.
Ultrathin films were prepared with cationic poly(allylamine hydrochloride) (PAH) and two anionic polysaccharides, iota- and lambda-carrageenan, of similar chemical composition but different conformations using the layer-by-layer (LbL) technique. The study of aqueous solutions of carrageenans confirms that iota-carrageenan is at room temperature in helical conformation while lambda-carrageenan is in random coil conformation. Characterization of the multilayers by ellipsometry, circular dichroism, and AFM revealed that iota-carrageenan keeps its helical conformation within the films while lambda-carrageenan chains are in random coil conformation. Investigation of the mechanical properties of the films by performing nanoindentation experiments using force spectroscopy showed clear differences between the two films based on carrageenans of different conformations.
使用层层（LbL）技术，用阳离子聚（烯丙胺盐酸盐）（PAH）和两种化学组成相似但构象不同的阴离子多糖——ι-卡拉胶和λ-卡拉胶制备了超薄膜。对卡拉胶水溶液的研究证实，ι-卡拉胶在室温下呈螺旋构象，而λ-卡拉胶呈无规卷曲构象。通过椭偏仪、圆二色性和原子力显微镜对多层膜进行表征，结果表明，ι-卡拉胶在薄膜中保持其螺旋构象，而λ-卡拉胶链呈无规卷曲构象。通过使用力谱进行纳米压痕实验来研究薄膜的力学性能，结果表明，基于不同构象的卡拉胶制成的两种薄膜之间存在明显差异。
