微波辅助从草木犀中提取香豆素及相关化合物，作为索氏提取法和超声辅助提取法的替代方法。

Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas as an alternative to Soxhlet and ultrasound-assisted extraction.

作者信息

Martino Emanuela, Ramaiola Ilaria, Urbano Mariangela, Bracco Francesco, Collina Simona

机构信息

Department of Territorial Ecology and Enviroment University of Pavia, Via S. Epifanio, 14-27100 Pavia, Italy.

出版信息

J Chromatogr A. 2006 Sep 1;1125(2):147-51. doi: 10.1016/j.chroma.2006.05.032.

DOI:10.1016/j.chroma.2006.05.032

PMID:16769080

Abstract

Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 degrees C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.

摘要

为测定草木犀花顶部香豆素、邻香豆酸和草木犀酸的含量，对索氏提取法、超声辅助提取法（USAE）和封闭系统中的微波辅助提取法（MAE）进行了研究。采用适当的高效液相色谱法对提取物进行分析。证明了提取和色谱分析的重现性。考虑到提取产率、成本和时间，我们研究了提取变量对上述化合物产率的影响。微波辅助提取法（50% v/v乙醇水溶液，50℃下两个5分钟的加热循环）取得了更好的结果。因此，基于提取产率/提取时间的比率，我们提出微波辅助提取法是最有效的方法。