Kasi Phanindra Babu, Molnár Kinga, László Lajos, Kotormán Márta
Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary.
Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University of Sciences, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary.
Biol Futur. 2021 Sep;72(3):367-372. doi: 10.1007/s42977-021-00086-0. Epub 2021 May 4.
The extracts of 7 herbs were screened and compared for their functional ability to inhibit the aggregation of trypsin as an appropriate model protein for in vitro fibrillation in aqueous ethanol at pH 7.0. Turbidity measurements, total phenolic content determination, aggregation kinetics, Congo red binding assay as well as transmission electron microscopy were used to analyse the inhibition of amyloid fibril formation. This correlated with the total phenolic content of the herb extracts. The peppermint extract proved to be the most potent anti-amyloidogenic agent. Results showed that the peppermint extract exerted dose-dependent inhibitory effect on trypsin fibril formation.
对7种草药提取物进行了筛选和比较，考察它们在pH 7.0的乙醇水溶液中抑制胰蛋白酶聚集的功能能力，胰蛋白酶是体外纤维化合适的模型蛋白。通过浊度测量、总酚含量测定、聚集动力学、刚果红结合试验以及透射电子显微镜来分析对淀粉样纤维形成的抑制作用。这与草药提取物的总酚含量相关。薄荷提取物被证明是最有效的抗淀粉样生成剂。结果表明，薄荷提取物对胰蛋白酶纤维形成具有剂量依赖性抑制作用。
