Ma Montesinos-Cisneros Rosa, Ortega Jaime, Guzmán Roberto, Tejeda-Mansir Armando
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Avenida IPN No. 2508, Mexico, DF 07360, Mexico.
Bioprocess Biosyst Eng. 2006 Jul;29(2):91-8. doi: 10.1007/s00449-006-0055-2. Epub 2006 Jun 13.
Breakthrough performance of linear-DNA adsorption on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. System dispersion curves showed the absence of flow non-idealities in the experimental arrangement. Breakthrough curves were not significantly affected by flow-rate or inlet solution concentration. In the theoretical analysis a model was integrated by the serial coupling of the membrane transport model and the system dispersion model. A transport model that considers finite kinetic rate and column dispersed flow was used in the study. A simplex optimization routine coupled to the solution of the partial differential model equations was employed to estimate the maximum adsorption capacity constant, the equilibrium desorption constant and the forward interaction rate-constant, which are the parameters of the membrane transport model. Through this approach a good prediction of the adsorption phenomena is obtained for inlet concentrations and flow rates greater than 0.2 mg/ml and 0.16 ml/min.
采用间歇式和固定床系统，从理论和实验两方面研究了线性DNA在离子交换膜柱上的突破性吸附性能。系统分散曲线表明实验装置中不存在流动非理想性。穿透曲线不受流速或入口溶液浓度的显著影响。在理论分析中，通过膜传输模型和系统分散模型的串联耦合建立了一个模型。本研究采用了考虑有限动力学速率和柱分散流的传输模型。采用与偏微分模型方程解耦合的单纯形优化程序来估计膜传输模型的参数，即最大吸附容量常数、平衡解吸常数和正向相互作用速率常数。通过这种方法，对于入口浓度大于0.2mg/ml和流速大于0.16ml/min的情况，能够很好地预测吸附现象。
