Montesinos-Cisneros Rosa Maria, Lucero-Acuña Armando, Ortega Jaime, Guzmán Roberto, Tejeda-Mansir Armando
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN (Centro de Investigacion y de Estudios Avanzados-Instituto Politécnico Nacional), Mexico City, D.F. (Distrito Federal), CP 07360, Mexico.
Biotechnol Appl Biochem. 2007 Oct;48(Pt 2):117-25. doi: 10.1042/ba20060166.
Protein adsorption of large proteins on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. Thyroglobulin was used as the model protein. The study strongly suggests that part of the protein is physically retained inside the column during frontal mode operation. These experimental results were used to obtain a filtration function of the chromatographic system. In the theoretical analysis of the frontal protein adsorption, a model was integrated by the serial coupling of the membrane-transport model, the filtration model and the system-dispersion model. Two different techniques were employed in the estimation of the maximum adsorption capacity, the equilibrium desorption constant and the forward interaction rate constant, which are the parameters of the membrane-transport model. The fit of the model to the experimental data was not possible using the equilibrium parameters obtained in the batch experiments. The parameter estimation using a simplex optimization routine coupled to the solution of the partial differential model equations yields full prediction of the adsorption phenomena.
使用间歇式和固定床系统对大蛋白质在离子交换膜柱上的蛋白质吸附进行了理论和实验研究。以甲状腺球蛋白作为模型蛋白。该研究有力地表明，在前沿模式操作期间，部分蛋白质被物理保留在柱内。这些实验结果被用于获得色谱系统的过滤函数。在前沿蛋白质吸附的理论分析中，通过膜传输模型、过滤模型和系统扩散模型的串联耦合整合了一个模型。采用了两种不同的技术来估计最大吸附容量、平衡解吸常数和正向相互作用速率常数，这些都是膜传输模型的参数。使用在间歇实验中获得的平衡参数，无法使模型与实验数据拟合。使用与偏微分模型方程的解耦合的单纯形优化程序进行参数估计，可以全面预测吸附现象。
