Diehl Peter, Naal Florian-Dominique, Schauwecker Johannes, Steinhauser Erwin, Milz Stefan, Gollwitzer Hans, Mittelmeier Wolfram
Klinik für Orthopädie und Sportorthopädie, Klinikum rechts der Isar der Technischen Universität München, Deutschland.
Biomed Tech (Berl). 2006 Apr;51(1):8-14. doi: 10.1515/BMT.2006.003.
Reconstruction of bone defects due to malignant tumors can be realized by several methods. Up to now, two methods, irradiation and autoclaving, are available for extracorporeally devitalizing resected tumor-bearing osteochondral segments. Previous investigations have shown that human normal and tumor cells in culture were irreversibly impaired when subjected to extracorporeal high hydrostatic pressure (HHP) of 350 MPa. The aim of this study was to examine the biomechanical and immunohistochemical properties of cartilage after exposure to HHP MATERIALS AND METHODS: Osteochondral segments of bovine femoral condyles were exposed to pressure of 300 and 600 MPa (n=20 each). Biomechanical and biological properties of untreated and treated segments were evaluated by repetitive ball indention testing and immunohistochemical labelling aggrecan, link protein and collagen II. The contralateral segments served as untreated control.
No significant alterations concerning stiffness and relaxation of osteochondral segments even after 600 MPa were observed. Immunohistochemically, staining was positive in all cases and no differences in the labeling pattern of proteoglycanes were observed between untreated and HHP-treated specimens.
These findings give hope that HHP eventually will be used as a new gentle way of treating resected cartilage and bone without alteration of biomechanical properties to inactivate tumor cells in order to allow autologous reimplantation.
恶性肿瘤导致的骨缺损可通过多种方法修复。目前，有两种方法，即辐照和高压灭菌，可用于体外灭活切除的含肿瘤骨软骨段。先前的研究表明，体外350兆帕的高静水压力（HHP）可使培养的人正常细胞和肿瘤细胞受到不可逆的损伤。本研究的目的是检测暴露于HHP后软骨的生物力学和免疫组化特性。材料与方法：将牛股骨髁的骨软骨段分别暴露于300兆帕和600兆帕的压力下（每组n = 20）。通过重复球压痕试验以及对聚集蛋白聚糖、连接蛋白和胶原蛋白II进行免疫组化标记，评估未处理和处理后骨软骨段的生物力学和生物学特性。对侧骨软骨段作为未处理对照。
即使在600兆帕压力处理后，骨软骨段的硬度和松弛度也未观察到明显变化。免疫组化结果显示，所有病例染色均为阳性，未处理标本与HHP处理标本之间蛋白聚糖的标记模式未观察到差异。
这些研究结果带来了希望，即HHP最终可能会成为一种新的温和方法，用于处理切除的软骨和骨，且不改变其生物力学特性，以灭活肿瘤细胞，从而实现自体再植入。
