Naal Florian D, Schauwecker Johannes, Steinhauser Erwin, Milz Stefan, von Knoch Fabian, Mittelmeier Wolfram, Diehl Peter
Department of Orthopaedic Surgery, Schulthess Clinic, 8008 Zurich, Switzerland.
J Biomed Mater Res B Appl Biomater. 2008 Oct;87(1):19-25. doi: 10.1002/jbm.b.31059.
Meniscal allograft processing procedures, in particular gamma irradiation, deteriorate the biomechanical and biological properties of the transplanted tissue. High hydrostatic pressure (HHP) treatment, widely used in food technology to inactivate microorganisms while preserving natural compounds, might serve as a gentle alternative to gamma irradiation in the processing of meniscal allografts. We therefore investigated the effects of HHP treatment on the biomechanical and immunohistochemical properties of meniscal cartilage. Specimens of bovine menisci were treated with HHP for 10 min (20 degrees C) at 300 MPa and 600 MPa. Untreated control samples were left at room temperature and ambient pressure. We performed repetitive cycling indentation-tests to assess the biomechanical properties-in particular the viscoelastic behavior-of HHP treated and untreated meniscal specimens. Immunohistochemical analysis for collagens type I, II, and III and for the proteoglycans versican, aggrecan and for link-protein was performed by immunolabeling cross-sections of untreated and at 600 MPa HHP treated specimens. Comparing untreated and HHP treated meniscal specimens there were no significant differences for all tested biomechanical parameters. All cross-sections of untreated and HHP treated specimens stained positive for the collagens and proteoglycans. We demonstrated that meniscal cartilage can be treated by HHP at levels as high as 600 MPa without affection of the biomechanical and immunochistochemical properties. Therefore, HHP treatment might serve as a gentle alternative to gamma irradiation in the processing of meniscal allografts. Further research is necessary to verificate the present results in vivo.
半月板同种异体移植物的处理程序，尤其是伽马射线照射，会使移植组织的生物力学和生物学特性恶化。高静水压（HHP）处理在食品技术中广泛用于灭活微生物同时保留天然化合物，在半月板同种异体移植物处理中可能是伽马射线照射的一种温和替代方法。因此，我们研究了HHP处理对半月板软骨生物力学和免疫组织化学特性的影响。牛半月板标本在300MPa和600MPa下于20℃用HHP处理10分钟。未处理的对照样品置于室温及常压下。我们进行了重复循环压痕试验以评估HHP处理和未处理的半月板标本的生物力学特性，特别是粘弹性行为。通过对未处理和600MPa HHP处理标本的横截面进行免疫标记，对I型、II型和III型胶原蛋白以及多功能蛋白聚糖、聚集蛋白聚糖和连接蛋白进行免疫组织化学分析。比较未处理和HHP处理的半月板标本，所有测试的生物力学参数均无显著差异。未处理和HHP处理标本的所有横截面的胶原蛋白和蛋白聚糖染色均呈阳性。我们证明半月板软骨可以在高达600MPa的水平下用HHP处理而不影响其生物力学和免疫组织化学特性。因此，HHP处理在半月板同种异体移植物处理中可能是伽马射线照射的一种温和替代方法。需要进一步研究以在体内验证目前的结果。
