Shafey T M, Al-Batshan H A, Shalaby M I, Ghannam M M
Department of Animal Production, King Saud University, Riyadh, Saudi Arabia.
Electromagn Biol Med. 2006;25(2):87-96. doi: 10.1080/15368370600718994.
Eggs from a layer-type breeder flock (Baladi, King Saud University) between 61 and 63 weeks of age were used in 3 trials to study the effects of electric field (EF) during incubation on the internal temperature of incubation, and eggs and hemoglobin (Hb) dielectric of chicken embryos at 18 days of age. Dielectric relative permittivity (epsilon') and conductivity (sigma) of Hb were examined in the range of frequency from 20 to 100 kHz. The values of dielectric increment (Deltaepsilon') and the relaxation times (tau) of Hb molecules were calculated. The internal temperature of eggs was measured in empty (following the removal of egg contents) and fertilized eggs in trials 1 and 2, respectively. The level of the EF was 30 kV/m, 60 Hz. EF incubation of embryos influenced the temperature of incubation and electrical properties of Hb molecules and did not influence the temperature of incubation and internal environment of eggs when empty eggs were incubated. EF incubation of fertilized eggs significantly raised the temperature of incubation, egg air cell, and at the surface of the egg yolk by approximately 0.09, 0.60, and 0.61 degrees F, respectively and Hb epsilon', sigma, Deltaepsilon', and tau as a function of the range of frequency of 20 to 100 kHz when compared with their counterparts of the control group. It was concluded that the exposure of fertilized chicken eggs to EF of 30 kV/m, 60 Hz, during incubation altered dielectric properties of Hb and that probably affected cell to cell communication and created the right environment for enhancing the growing process and heat production of embryos consequently increasing the temperature of the internal environment of the egg, and incubation.
在3项试验中，使用了来自61至63周龄的蛋用型种鸡群（沙特国王大学巴拉迪品种）的鸡蛋，以研究孵化期间电场（EF）对孵化内部温度、18日龄鸡胚的鸡蛋和血红蛋白（Hb）介电性能的影响。在20至100 kHz的频率范围内检测了Hb的相对介电常数（ε'）和电导率（σ）。计算了Hb分子的介电增量（Δε'）和弛豫时间（τ）。在试验1和试验2中，分别测量了空蛋（去除蛋内容物后）和受精卵的内部温度。电场水平为30 kV/m，60 Hz。胚胎的电场孵化影响了孵化温度和Hb分子的电性能，而空蛋孵化时电场对孵化温度和鸡蛋内部环境没有影响。与对照组相比，受精卵的电场孵化使孵化温度、蛋气室和蛋黄表面温度分别显著升高约0.09、0.60和0.61华氏度，并且在20至100 kHz频率范围内，Hb的ε'、σ、Δε'和τ也发生了变化。得出的结论是，孵化期间将受精鸡蛋暴露于30 kV/m、60 Hz的电场中会改变Hb的介电性能，这可能影响细胞间通讯，并为促进胚胎生长过程和产热创造合适的环境，从而提高蛋的内部环境温度和孵化温度。
