将同位素碳从非葡萄糖底物掺入脑糖原中。
Incorporation of isotopic carbon into cerebral glycogen from non-glucose substrates.
作者信息
Phillips M E, Coxon R V
出版信息
Biochem J. 1975 Jan;146(1):185-9. doi: 10.1042/bj1460185.
Abstract
- Measurable incorporation of radioactive carbon from [U-14C]pyruvate, [U-14C]-glutamate and [14C]bicarbonate into the glycogen synthesized by brain slices in vitro was demonstrated. 2. The fructose diphosphatase activity of guinea-pig brain was determined and found to be about 0.03 mumol of substrate degraded/min per g of fresh tissue. 3. The specific radioactivity of the glucose carbon from glycogen relative to that of the precursor added to the incubation medium gave approximate values of 0.195 for glucose, 0.006 for pyruvate, 0.039 for glutamate and 0.001 for bicarbonate.
摘要
- 已证实在体外由脑切片合成的糖原中可测量到放射性碳从[U-14C]丙酮酸、[U-14C]谷氨酸盐和[14C]碳酸氢盐中掺入。2. 测定了豚鼠脑的果糖二磷酸酶活性，发现其为每克新鲜组织每分钟降解约0.03微摩尔底物。3. 糖原中葡萄糖碳的比放射性相对于添加到孵育培养基中的前体的比放射性，葡萄糖约为0.195，丙酮酸为0.006，谷氨酸盐为0.039，碳酸氢盐为0.001。
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