关于在肝脏和肌肉中存在一条无需以6-磷酸葡萄糖作为中间产物就能将葡萄糖转化为糖原的途径的研究。
Studies on the existence of a pathway in liver and muscle for the conversion of glucose into glycogen without glucose 6-phosphate as an intermediate.
作者信息
Antony G J, Srinivasan I, Williams H R, Landau B R
出版信息
Biochem J. 1969 Feb;111(4):453-9. doi: 10.1042/bj1110453.
Mixtures of (14)C-labelled glucose plus pyruvate were incubated either with rat diaphragm or slices of rat liver. Incorporation of glucose carbon into glycogen was compared with its incorporation into glucose 6-phosphate relative to the incorporation of pyruvate carbon into these metabolic products. There was no preferential incorporation of glucose carbon relative to pyruvate carbon into glycogen compared with glucose 6-phosphate in the liver slices, but there was in diaphragm. On the assumption that glucose 6-phosphate is a necessary intermediate in the conversion of pyruvate carbon into glycogen, this is evidence for the existence in muscle, but not in liver, of more than one pool of glucose 6-phosphate or of a pathway from glucose to glycogen without glucose 6-phosphate as an intermediate. Galactose carbon, relative to pyruvate carbon, was preferentially incorporated into liver glycogen, so that a substrate converted in liver into glycogen without glucose 6-phosphate as an intermediate could be detected by this approach.
将含有¹⁴C标记的葡萄糖与丙酮酸的混合物分别与大鼠膈肌或大鼠肝脏切片一起孵育。相对于丙酮酸碳掺入这些代谢产物的情况，比较葡萄糖碳掺入糖原和葡萄糖-6-磷酸的情况。在肝脏切片中，与葡萄糖-6-磷酸相比，葡萄糖碳相对于丙酮酸碳掺入糖原并无优先性，但在膈肌中存在优先性。假设葡萄糖-6-磷酸是丙酮酸碳转化为糖原过程中的必要中间体，这证明肌肉中存在不止一个葡萄糖-6-磷酸池或存在一条不以葡萄糖-6-磷酸为中间体的从葡萄糖到糖原的途径，而肝脏中不存在。相对于丙酮酸碳，半乳糖碳优先掺入肝脏糖原，因此通过这种方法可以检测到在肝脏中转化为糖原而不以葡萄糖-6-磷酸为中间体的底物。
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