Xu Han, Miao Xiaoling, Wu Qingyu
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 10084, PR China.
J Biotechnol. 2006 Dec 1;126(4):499-507. doi: 10.1016/j.jbiotec.2006.05.002.
The aim of the study was to obtain high quality biodiesel production from a microalga Chlorella protothecoids through the technology of transesterification. The technique of metabolic controlling through heterotrophic growth of C. protothecoides was applied, and the heterotrophic C. protothecoides contained the crude lipid content of 55.2%. To increase the biomass and reduce the cost of alga, corn powder hydrolysate instead of glucose was used as organic carbon source in heterotrophic culture medium in fermenters. The result showed that cell density significantly increased under the heterotrophic condition, and the highest cell concentration reached 15.5 g L(-1). Large amount of microalgal oil was efficiently extracted from the heterotrophic cells by using n-hexane, and then transmuted into biodiesel by acidic transesterification. The biodiesel was characterized by a high heating value of 41 MJ kg(-1), a density of 0.864 kg L(-1), and a viscosity of 5.2 x 10(-4) Pa s (at 40 degrees C). The method has great potential in the industrial production of liquid fuel from microalga.
本研究的目的是通过酯交换技术从原球藻属微藻中获得高质量的生物柴油。采用了通过原球藻异养生长进行代谢控制的技术，异养的原球藻粗脂肪含量为55.2%。为了增加生物量并降低藻类成本，在发酵罐的异养培养基中使用玉米粉水解产物代替葡萄糖作为有机碳源。结果表明，在异养条件下细胞密度显著增加，最高细胞浓度达到15.5 g L(-1)。使用正己烷从异养细胞中高效提取了大量微藻油，然后通过酸性酯交换将其转化为生物柴油。该生物柴油的高热值为41 MJ kg(-1)，密度为0.864 kg L(-1)，粘度为5.2×10(-4) Pa s（在40℃时）。该方法在微藻液体燃料的工业生产中具有巨大潜力。
