Reusch C, Vochezer R, Weschta E
Department of Veterinary Internal Medicine, University of Munich, Germany.
Zentralbl Veterinarmed A. 1991 Mar;38(2):90-8. doi: 10.1111/j.1439-0442.1991.tb00988.x.
The activity of the urinary enzymes alanine aminopeptidase (AAP; EC 3.4.11.2) and N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) was measured after eliminating distorting factors in ten healthy dogs on three consecutive days in order to determine inter- and intra-individual variability. All the animals were being housed and fed in the same way. The urine (2nd morning urine) was collected between 8:30 and 10:00 a.m. by means of ultrasound-controlled cystocentesis. Our comparative measurements of native and gel-filtered urine showed an increase in enzyme activity in all of the samples in the case of AAP and in most of the samples in the case of NAG, thus proving the existence in the dog of AAP and NAG urinary enzyme inhibitors. The large inter- and intra-individual AAP and NAG fluctuation ranges were reduced considerably by relating enzyme activity to urine creatinine concentration. The provisional upper limit of the AAP reference range is 6.3 U/g creatinine (90% percentile), that of NAG 6.2 U/g creatinine (90% percentile). The AAP and NAG enzyme activities remained constant in gel-filtered samples kept at 4 degrees C for at least 5 days, in those kept at -18 degrees C for at least 4 weeks.
为了确定个体间和个体内的变异性，在连续三天消除干扰因素后，对10只健康犬的尿酶丙氨酸氨基肽酶（AAP；EC 3.4.11.2）和N-乙酰-β-D-氨基葡萄糖苷酶（NAG；EC 3.2.1.30）的活性进行了测定。所有动物的饲养和喂食方式相同。上午8:30至10:00通过超声引导膀胱穿刺术收集尿液（第二天晨尿）。我们对未处理尿液和凝胶过滤尿液的对比测量显示，在所有AAP样本以及大多数NAG样本中，酶活性均有所增加，从而证明犬体内存在AAP和NAG尿酶抑制剂。通过将酶活性与尿肌酐浓度相关联，个体间和个体内AAP和NAG的大幅波动范围显著减小。AAP参考范围的临时上限为6.3 U/g肌酐（第90百分位数），NAG为6.2 U/g肌酐（第90百分位数）。在4℃保存至少5天以及在-18℃保存至少4周的凝胶过滤样本中，AAP和NAG酶活性保持恒定。
