一种通过Paip2与eIF4G竞争结合多聚腺苷酸结合蛋白（PABP）来实现翻译抑制的机制。
A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) binding.
作者信息
Karim Muhammad M, Svitkin Yuri V, Kahvejian Avak, De Crescenzo Gregory, Costa-Mattioli Mauro, Sonenberg Nahum
机构信息
Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, QC, Canada H3G 1Y6.
出版信息
Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9494-9. doi: 10.1073/pnas.0603701103. Epub 2006 Jun 13.
Abstract
The eukaryotic mRNA 3' poly(A) tail and the 5' cap cooperate to synergistically enhance translation. This interaction is mediated by the cap-binding protein eIF4E, the poly(A) binding protein (PABP), and eIF4G, a scaffolding protein that bridges between eIF4E and PABP to bring about the circularization of the mRNA. The translational repressor, Paip2 (PABP-interacting protein 2), inhibits translation by promoting the dissociation of PABP from poly(A). Here we report on the existence of an alternative mechanism by which Paip2 inhibits translation by competing with eIF4G for binding to PABP. We demonstrate that Paip2 can abrogate the translational activity of PABP, which is tethered to the 3' end of the mRNA. Thus, Paip2 can inhibit translation by a previously unrecognized mechanism, which is independent of its ability to disrupt PABP-poly(A) interaction.
摘要
真核生物信使核糖核酸（mRNA）的3' 聚腺苷酸（poly(A)）尾巴和5' 帽协同作用以增强翻译。这种相互作用由帽结合蛋白eIF4E、聚腺苷酸结合蛋白（PABP）和eIF4G介导，eIF4G是一种支架蛋白，它在eIF4E和PABP之间架桥，使mRNA环化。翻译抑制因子Paip2（PABP相互作用蛋白2）通过促进PABP与聚腺苷酸的解离来抑制翻译。在此我们报告存在一种替代机制，通过该机制Paip2通过与eIF4G竞争结合PABP来抑制翻译。我们证明Paip2可以消除与mRNA 3' 端相连的PABP的翻译活性。因此，Paip2可以通过一种以前未被认识的机制抑制翻译，该机制与其破坏PABP - 聚腺苷酸相互作用的能力无关。
相似文献
1
A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) binding.一种通过Paip2与eIF4G竞争结合多聚腺苷酸结合蛋白（PABP）来实现翻译抑制的机制。
Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9494-9. doi: 10.1073/pnas.0603701103. Epub 2006 Jun 13.
2
Dual interactions of the translational repressor Paip2 with poly(A) binding protein.翻译阻遏蛋白Paip2与聚腺苷酸结合蛋白的双重相互作用
Mol Cell Biol. 2001 Aug;21(15):5200-13. doi: 10.1128/MCB.21.15.5200-5213.2001.
3
[Translational control by the poly(A) binding protein: a check for mRNA integrity].聚腺苷酸结合蛋白对翻译的调控：对mRNA完整性的检查
Mol Biol (Mosk). 2006 Jul-Aug;40(4):684-93.
4
Regulation of poly(A) binding protein function in translation: Characterization of the Paip2 homolog, Paip2B.翻译过程中多聚腺苷酸结合蛋白功能在翻译中的调控：Paip2 同源物 Paip2B 的特性分析。
RNA. 2006 Aug;12(8):1556-68. doi: 10.1261/rna.106506. Epub 2006 Jun 27.
5
Translational repression by a novel partner of human poly(A) binding protein, Paip2.人类聚腺苷酸结合蛋白的新型伴侣Paip2介导的翻译抑制
Mol Cell. 2001 Jan;7(1):205-16. doi: 10.1016/s1097-2765(01)00168-x.
6
Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding.病毒和细胞 mRNA 特异性激活因子利用 PABP 和 eIF4G 来促进帽结合下游的翻译起始。
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6310-6315. doi: 10.1073/pnas.1610417114. Epub 2017 May 30.
7
Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP.神经RNA结合蛋白Musashi1通过与真核翻译起始因子4G（eIF4G）竞争多聚腺苷酸结合蛋白（PABP）来抑制翻译起始。
J Cell Biol. 2008 May 19;181(4):639-53. doi: 10.1083/jcb.200708004.
8
The bent conformation of poly(A)-binding protein induced by RNA-binding is required for its translational activation function.RNA结合诱导的聚腺苷酸结合蛋白的弯曲构象是其翻译激活功能所必需的。
RNA Biol. 2017 Mar 4;14(3):370-377. doi: 10.1080/15476286.2017.1280224. Epub 2017 Jan 17.
9
Competitive and noncompetitive binding of eIF4B, eIF4A, and the poly(A) binding protein to wheat translation initiation factor eIFiso4G.eIF4B、eIF4A 和 poly(A) 结合蛋白与小麦翻译起始因子 eIFiso4G 的竞争性和非竞争性结合。
Biochemistry. 2010 Sep 28;49(38):8251-65. doi: 10.1021/bi1008529.
10
Translational silencing of ceruloplasmin requires the essential elements of mRNA circularization: poly(A) tail, poly(A)-binding protein, and eukaryotic translation initiation factor 4G.铜蓝蛋白的翻译沉默需要mRNA环化的基本要素：聚腺苷酸尾、聚腺苷酸结合蛋白和真核生物翻译起始因子4G。
Mol Cell Biol. 2001 Oct;21(19):6440-9. doi: 10.1128/MCB.21.19.6440-6449.2001.
引用本文的文献
1
Structured and disordered regions of Ataxin-2 contribute differently to the specificity and efficiency of mRNP granule formation.Ataxin-2 的结构域和无规则区域对 mRNP 颗粒形成的特异性和效率有不同的贡献。
PLoS Genet. 2024 May 20;20(5):e1011251. doi: 10.1371/journal.pgen.1011251. eCollection 2024 May.
2
dCas13-mediated translational repression for accurate gene silencing in mammalian cells.dCas13介导的翻译抑制用于在哺乳动物细胞中实现精确的基因沉默。
Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7.
3
Circular RNAs, Noncoding RNAs, and N6-methyladenosine Involved in the Development of MAFLD.参与MAFLD发生发展的环状RNA、非编码RNA和N6-甲基腺苷
Noncoding RNA. 2024 Feb 5;10(1):11. doi: 10.3390/ncrna10010011.
4
The flip-flop configuration of the PABP-dimer leads to switching of the translation function.PABP-二聚体的翻转型构导致翻译功能的切换。
Nucleic Acids Res. 2022 Jan 11;50(1):306-321. doi: 10.1093/nar/gkab1205.
5
A forward genetic screen identifies modifiers of rocaglate responsiveness.正向遗传筛选鉴定罗卡胍反应性的修饰因子。
Sci Rep. 2021 Sep 16;11(1):18516. doi: 10.1038/s41598-021-97765-8.
6
Translation of yes-associated protein (YAP) was antagonized by its circular RNA via suppressing the assembly of the translation initiation machinery.通过抑制翻译起始机制的组装，YAP 的环状 RNA 拮抗了其翻译。
Cell Death Differ. 2019 Dec;26(12):2758-2773. doi: 10.1038/s41418-019-0337-2. Epub 2019 May 15.
7
Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila.Paip2 在活性启动子处与 Cbp80 合作，并参与果蝇 RNA 聚合酶 II 的磷酸化。
FEBS Lett. 2019 May;593(10):1102-1112. doi: 10.1002/1873-3468.13391. Epub 2019 Apr 29.
8
Polyadenylate-binding protein-interacting proteins PAIP1 and PAIP2 affect translation termination.多聚腺苷酸结合蛋白相互作用蛋白 PAIP1 和 PAIP2 影响翻译终止。
J Biol Chem. 2019 May 24;294(21):8630-8639. doi: 10.1074/jbc.RA118.006856. Epub 2019 Apr 16.
9
Translational Control in Stem Cells.干细胞中的翻译控制
Front Genet. 2019 Jan 15;9:709. doi: 10.3389/fgene.2018.00709. eCollection 2018.
10
Dynamic interaction of poly(A)-binding protein with the ribosome.多聚（A）结合蛋白与核糖体的动态相互作用。
Sci Rep. 2018 Nov 28;8(1):17435. doi: 10.1038/s41598-018-35753-1.
本文引用的文献
1
Comparative peptide binding studies of the PABC domains from the ubiquitin-protein isopeptide ligase HYD and poly(A)-binding protein. Implications for HYD function.泛素-蛋白质异肽连接酶HYD和聚腺苷酸结合蛋白的PABC结构域的肽结合比较研究。对HYD功能的影响。
J Biol Chem. 2006 May 19;281(20):14376-82. doi: 10.1074/jbc.M600307200. Epub 2006 Mar 22.
2
The DAZL family proteins are PABP-binding proteins that regulate translation in germ cells.DAZL家族蛋白是与多聚腺苷酸结合蛋白（PABP）结合的蛋白，可调节生殖细胞中的翻译过程。
EMBO J. 2005 Jul 20;24(14):2656-66. doi: 10.1038/sj.emboj.7600738. Epub 2005 Jul 7.
3
Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms.哺乳动物聚腺苷酸结合蛋白是一种真核生物翻译起始因子，它通过多种机制发挥作用。
Genes Dev. 2005 Jan 1;19(1):104-13. doi: 10.1101/gad.1262905.
4
An efficient system for cap- and poly(A)-dependent translation in vitro.一种用于体外帽依赖性和多聚腺苷酸依赖性翻译的高效系统。
Methods Mol Biol. 2004;257:155-70. doi: 10.1385/1-59259-750-5:155.
5
Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.聚腺苷酸结合蛋白：用于基因表达转录后调控的多功能支架
Genome Biol. 2003;4(7):223. doi: 10.1186/gb-2003-4-7-223. Epub 2003 Jul 1.
6
The mRNA closed-loop model: the function of PABP and PABP-interacting proteins in mRNA translation.信使核糖核酸闭环模型：聚腺苷酸结合蛋白（PABP）及与PABP相互作用蛋白在信使核糖核酸翻译中的功能
Cold Spring Harb Symp Quant Biol. 2001;66:293-300. doi: 10.1101/sqb.2001.66.293.
7
A novel role of the mammalian GSPT/eRF3 associating with poly(A)-binding protein in Cap/Poly(A)-dependent translation.哺乳动物GSPT/eRF3与聚腺苷酸结合蛋白在帽状结构/聚腺苷酸依赖性翻译中关联的新作用。
J Biol Chem. 2002 Dec 27;277(52):50286-92. doi: 10.1074/jbc.M203029200. Epub 2002 Oct 14.
8
Generation of multiple isoforms of eukaryotic translation initiation factor 4GI by use of alternate translation initiation codons.通过使用可变翻译起始密码子产生真核生物翻译起始因子4GI的多种同工型。
Mol Cell Biol. 2002 Jul;22(13):4499-511. doi: 10.1128/MCB.22.13.4499-4511.2002.
9
Paip1 interacts with poly(A) binding protein through two independent binding motifs.Paip1通过两个独立的结合基序与聚腺苷酸结合蛋白相互作用。
Mol Cell Biol. 2002 Jun;22(11):3769-82. doi: 10.1128/MCB.22.11.3769-3782.2002.
Zotero 插件