• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

PABP-二聚体的翻转型构导致翻译功能的切换。

The flip-flop configuration of the PABP-dimer leads to switching of the translation function.

机构信息

Department of Life Sciences, Pohang University of Science and Technology, Nam-gu, Pohang 37673, Republic of Korea.

School of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Nam-gu, Pohang 37673, Republic of Korea.

出版信息

Nucleic Acids Res. 2022 Jan 11;50(1):306-321. doi: 10.1093/nar/gkab1205.

DOI:10.1093/nar/gkab1205
PMID:34904669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8754640/
Abstract

Poly(A)-binding protein (PABP) is a translation initiation factor that interacts with the poly(A) tail of mRNAs. PABP bound to poly(A) stimulates translation by interacting with the eukaryotic initiation factor 4G (eIF4G), which brings the 3' end of an mRNA close to its 5' m7G cap structure through consecutive interactions of the 3'-poly(A)-PABP-eIF4G-eIF4E-5' m7G cap. PABP is a highly abundant translation factor present in considerably larger quantities than mRNA and eIF4G in cells. However, it has not been elucidated how eIF4G, present in limited cellular concentrations, is not sequestered by mRNA-free PABP, present at high cellular concentrations, but associates with PABP complexed with the poly(A) tail of an mRNA. Here, we report that RNA-free PABPs dimerize with a head-to-head type configuration of PABP, which interferes in the interaction between PABP and eIF4G. We identified the domains of PABP responsible for PABP-PABP interaction. Poly(A) RNA was shown to convert the PABP-PABP complex into a poly(A)-PABP complex, with a head-to-tail-type configuration of PABP that facilitates the interaction between PABP and eIF4G. Lastly, we showed that the transition from the PABP dimer to the poly(A)-PABP complex is necessary for the translational activation function.

摘要

聚腺苷酸化结合蛋白（PABP）是一种翻译起始因子，可与 mRNA 的 poly(A) 尾巴相互作用。与 poly(A) 结合的 PABP 通过与真核起始因子 4G（eIF4G）相互作用来刺激翻译，eIF4G 通过 poly(A)-PABP-eIF4G-eIF4E-5' m7G 帽结构的连续相互作用，使 mRNA 的 3' 端靠近其 5' m7G 帽结构。PABP 是一种高度丰富的翻译因子，在细胞中的含量远高于 mRNA 和 eIF4G。然而，目前还不清楚为什么在细胞中浓度有限的 eIF4G 不会被高细胞浓度下存在的无 mRNA 的 PABP 隔离，而是与与 mRNA 的 poly(A) 尾巴结合的 PABP 相关联。在这里，我们报告说无 RNA 的 PABP 以 PABP 头对头的类型构型二聚化，这会干扰 PABP 和 eIF4G 之间的相互作用。我们确定了 PABP 负责 PABP-PABP 相互作用的结构域。poly(A) RNA 显示出将 PABP-PABP 复合物转化为具有 PABP 头到尾类型构型的 poly(A)-PABP 复合物，这有利于 PABP 和 eIF4G 之间的相互作用。最后，我们表明从 PABP 二聚体到 poly(A)-PABP 复合物的转变是翻译激活功能所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/9cf287a1c33b/gkab1205fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/85f8965ed80b/gkab1205fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/5772e92a0c0e/gkab1205fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/d9eb9da82d1c/gkab1205fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/f42e43e17ae6/gkab1205fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/099e848e7938/gkab1205fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/f8f85e1fa405/gkab1205fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/9cf287a1c33b/gkab1205fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/85f8965ed80b/gkab1205fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/5772e92a0c0e/gkab1205fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/d9eb9da82d1c/gkab1205fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/f42e43e17ae6/gkab1205fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/099e848e7938/gkab1205fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/f8f85e1fa405/gkab1205fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f144/8754640/9cf287a1c33b/gkab1205fig7.jpg

相似文献

1
The flip-flop configuration of the PABP-dimer leads to switching of the translation function.PABP-二聚体的翻转型构导致翻译功能的切换。
Nucleic Acids Res. 2022 Jan 11;50(1):306-321. doi: 10.1093/nar/gkab1205.
2
Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding.病毒和细胞 mRNA 特异性激活因子利用 PABP 和 eIF4G 来促进帽结合下游的翻译起始。
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6310-6315. doi: 10.1073/pnas.1610417114. Epub 2017 May 30.
3
The bent conformation of poly(A)-binding protein induced by RNA-binding is required for its translational activation function.RNA结合诱导的聚腺苷酸结合蛋白的弯曲构象是其翻译激活功能所必需的。
RNA Biol. 2017 Mar 4;14(3):370-377. doi: 10.1080/15476286.2017.1280224. Epub 2017 Jan 17.
4
eIF4G, eIFiso4G, and eIF4B bind the poly(A)-binding protein through overlapping sites within the RNA recognition motif domains.真核生物翻译起始因子4G（eIF4G）、真核生物翻译起始因子异4G（eIFiso4G）和真核生物翻译起始因子4B（eIF4B）通过RNA识别基序结构域内的重叠位点与聚腺苷酸结合蛋白结合。
J Biol Chem. 2007 Aug 31;282(35):25247-58. doi: 10.1074/jbc.M702193200. Epub 2007 Jul 2.
5
A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) binding.一种通过Paip2与eIF4G竞争结合多聚腺苷酸结合蛋白（PABP）来实现翻译抑制的机制。
Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9494-9. doi: 10.1073/pnas.0603701103. Epub 2006 Jun 13.
6
Dynamic interaction of poly(A)-binding protein with the ribosome.多聚（A）结合蛋白与核糖体的动态相互作用。
Sci Rep. 2018 Nov 28;8(1):17435. doi: 10.1038/s41598-018-35753-1.
7
Translational silencing of ceruloplasmin requires the essential elements of mRNA circularization: poly(A) tail, poly(A)-binding protein, and eukaryotic translation initiation factor 4G.铜蓝蛋白的翻译沉默需要mRNA环化的基本要素：聚腺苷酸尾、聚腺苷酸结合蛋白和真核生物翻译起始因子4G。
Mol Cell Biol. 2001 Oct;21(19):6440-9. doi: 10.1128/MCB.21.19.6440-6449.2001.
8
Enhancement of IRES-mediated translation of the c-myc and BiP mRNAs by the poly(A) tail is independent of intact eIF4G and PABP.聚腺苷酸尾增强内部核糖体进入位点（IRES）介导的c-myc和BiP mRNA翻译，这一过程独立于完整的真核起始因子4G（eIF4G）和聚腺苷酸结合蛋白（PABP）。
Mol Cell. 2004 Sep 24;15(6):925-35. doi: 10.1016/j.molcel.2004.08.021.
9
Rotavirus NSP3 Is a Translational Surrogate of the Poly(A) Binding Protein-Poly(A) Complex.轮状病毒NSP3是聚腺苷酸结合蛋白-聚腺苷酸复合物的翻译替代物。
J Virol. 2015 Sep;89(17):8773-82. doi: 10.1128/JVI.01402-15. Epub 2015 Jun 10.
10
Epstein-Barr Virus Protein EB2 Stimulates Translation Initiation of mRNAs through Direct Interactions with both Poly(A)-Binding Protein and Eukaryotic Initiation Factor 4G.爱泼斯坦-巴尔病毒蛋白EB2通过与聚腺苷酸结合蛋白和真核起始因子4G直接相互作用刺激mRNA的翻译起始。
J Virol. 2018 Jan 17;92(3). doi: 10.1128/JVI.01917-17. Print 2018 Feb 1.

引用本文的文献

1
Participation of host cell proteins in inclusion bodies of non-segmented RNA virus infected cells: a molecular insight.宿主细胞蛋白参与非节段RNA病毒感染细胞的包涵体形成：分子层面的见解
Virol J. 2025 Aug 18;22(1):282. doi: 10.1186/s12985-025-02784-w.
2
Developing Vaccines in Pancreatic Adenocarcinoma: Trials and Tribulations.开发胰腺腺癌疫苗：试验与磨难。
Curr Oncol. 2024 Aug 23;31(9):4855-4884. doi: 10.3390/curroncol31090361.
3
The impact of mRNA poly(A) tail length on eukaryotic translation stages.mRNA 多聚（A）尾长度对真核翻译阶段的影响。

本文引用的文献

1
A phosphorylation-regulated eIF3d translation switch mediates cellular adaptation to metabolic stress.磷酸化调节的 eIF3d 翻译开关介导细胞适应代谢应激。
Science. 2020 Nov 13;370(6518):853-856. doi: 10.1126/science.abb0993.
2
Structure of a human 48 translational initiation complex.人源 48 翻译起始复合物的结构。
Science. 2020 Sep 4;369(6508):1220-1227. doi: 10.1126/science.aba4904.
3
Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase.多聚（A）核糖核蛋白结构的分子基础及 Pan2-Pan3 去腺苷酶的识别作用。
Nucleic Acids Res. 2024 Jul 22;52(13):7792-7808. doi: 10.1093/nar/gkae510.
4
FMRP Enhances the Translation of mRNA during Neuronal Differentiation.FMRP 增强神经元分化过程中 mRNA 的翻译。
Int J Mol Sci. 2023 Nov 14;24(22):16319. doi: 10.3390/ijms242216319.
5
The use of RNA-based treatments in the field of cancer immunotherapy.基于 RNA 的治疗方法在癌症免疫治疗领域的应用。
Mol Cancer. 2023 Jul 7;22(1):106. doi: 10.1186/s12943-023-01807-w.
6
The flavagline FL3 interferes with the association of Annexin A2 with the eIF4F initiation complex and transiently stimulates the translation of A2 mRNA.黄酮类化合物FL3干扰膜联蛋白A2与eIF4F起始复合物的结合，并短暂刺激A2 mRNA的翻译。
Front Cell Dev Biol. 2023 May 12;11:1094941. doi: 10.3389/fcell.2023.1094941. eCollection 2023.
7
Single-molecule visualization of mRNA circularization during translation.在翻译过程中单分子可视化 mRNA 环化。
Exp Mol Med. 2023 Feb;55(2):283-289. doi: 10.1038/s12276-023-00933-1. Epub 2023 Jan 31.
Cell. 2019 May 30;177(6):1619-1631.e21. doi: 10.1016/j.cell.2019.04.013. Epub 2019 May 16.
4
Structural and Functional Diversity of Plant Virus 3'-Cap-Independent Translation Enhancers (3'-CITEs).植物病毒3' - 非帽依赖型翻译增强子（3' - CITEs）的结构与功能多样性
Front Plant Sci. 2017 Nov 29;8:2047. doi: 10.3389/fpls.2017.02047. eCollection 2017.
5
Extensive translation of circular RNAs driven by N-methyladenosine.由N-甲基腺苷驱动的环状RNA的广泛翻译。
Cell Res. 2017 May;27(5):626-641. doi: 10.1038/cr.2017.31. Epub 2017 Mar 10.
6
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination.cryoSPARC：用于快速无监督低温电子显微镜结构测定的算法。
Nat Methods. 2017 Mar;14(3):290-296. doi: 10.1038/nmeth.4169. Epub 2017 Feb 6.
7
The bent conformation of poly(A)-binding protein induced by RNA-binding is required for its translational activation function.RNA结合诱导的聚腺苷酸结合蛋白的弯曲构象是其翻译激活功能所必需的。
RNA Biol. 2017 Mar 4;14(3):370-377. doi: 10.1080/15476286.2017.1280224. Epub 2017 Jan 17.
8
eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation.真核生物翻译起始因子3d（eIF3d）是一种mRNA帽结合蛋白，是特殊翻译起始所必需的。
Nature. 2016 Aug 4;536(7614):96-9. doi: 10.1038/nature18954. Epub 2016 Jul 27.
9
Comparative genetics. Systematic discovery of cap-independent translation sequences in human and viral genomes.比较遗传学。在人类和病毒基因组中系统发现非依赖性翻译序列。
Science. 2016 Jan 15;351(6270). doi: 10.1126/science.aad4939. Epub 2016 Jan 14.
10
Cap-dependent translation is mediated by 'RNA looping' rather than 'ribosome scanning'.帽依赖性翻译是由“RNA环化”介导的，而非“核糖体扫描”。
RNA Biol. 2016;13(1):1-5. doi: 10.1080/15476286.2015.1107700.