Lemus Dihadenys, Montoro Ernesto, Echemendía Miguel, Martin Anandi, Portaels Françoise, Palomino Juan Carlos
Instituto de Medicina Tropical 'Pedro Kouri', Autopista Novia del Mediodía Km 61/2, Apartado Postal 601, La Lisa, La Habana, Cuba.
Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
J Med Microbiol. 2006 Jul;55(Pt 7):861-863. doi: 10.1099/jmm.0.46540-0.
The nitrate reductase assay (NRA) was used as an alternative method for detection of resistance to the first-line antituberculous drugs isoniazid, rifampicin, ethambutol and streptomycin. A total of 320 strains of Mycobacterium tuberculosis were studied and the results compared with the proportion method (PM) on Löwenstein-Jensen medium. The mean time to obtain results was 10 days and the overall agreement between the NRA and PM was 98.8 %. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis strains in low-resource countries.
硝酸还原酶测定法（NRA）被用作检测对一线抗结核药物异烟肼、利福平、乙胺丁醇和链霉素耐药性的替代方法。共研究了320株结核分枝杆菌菌株，并将结果与在罗-琴培养基上的比例法（PM）进行比较。获得结果的平均时间为10天，NRA与PM之间的总体一致性为98.8%。NRA操作简便，是资源匮乏国家快速准确测定耐药结核分枝杆菌菌株的有用工具。
