幽门螺杆菌相关的氧化剂一氯胺可诱导潜伏感染爱泼斯坦-巴尔病毒（EBV）的胃上皮细胞中EBV重新激活。

Helicobacter pylori-associated oxidant monochloramine induces reactivation of Epstein-Barr virus (EBV) in gastric epithelial cells latently infected with EBV.

作者信息

Minoura-Etoh Junko, Gotoh Kazuyo, Sato Ryugo, Ogata Masao, Kaku Naomi, Fujioka Toshio, Nishizono Akira

出版信息

J Med Microbiol. 2006 Jul;55(Pt 7):905-911. doi: 10.1099/jmm.0.46580-0.

DOI:10.1099/jmm.0.46580-0

Abstract

To investigate the possibility of an interaction between two ubiquitous human pathogens, Helicobacter pylori and Epstein-Barr virus (EBV), the effect of monochloramine (NH2Cl), locally produced by H. pylori infection, on gastric epithelium latently infected with EBV was examined, by assessing the induction of EBV lytic infection. AGS cells harbouring latently infected EBV were used as the indicator of lytic change caused by NH2Cl treatment. Lytic infection, determined by morphological change and EA-D antigen expression, occurred immediately after treatment with in vitro-synthesized NH2Cl. Analysis of EBV infection in human gastric tissue revealed that out of 48 H. pylori-positive patients, 24 were positive for EBER-1, and 18 and 13 were positive for EBNA1 and LMP-1 antigen, respectively. The results suggest that H. pylori-associated NH2Cl induces EBV lytic conversion in gastric epithelium latently infected with EBV.

摘要

为了研究两种常见的人类病原体——幽门螺杆菌和爱泼斯坦-巴尔病毒（EBV）之间相互作用的可能性，通过评估EBV裂解感染的诱导情况，检测了幽门螺杆菌感染局部产生的一氯胺（NH2Cl）对潜伏感染EBV的胃上皮细胞的影响。携带潜伏感染EBV的AGS细胞被用作NH2Cl处理引起裂解变化的指标。用体外合成的NH2Cl处理后，立即出现了由形态变化和EA-D抗原表达所确定的裂解感染。对人胃组织中EBV感染的分析显示，在48例幽门螺杆菌阳性患者中，24例EBER-1呈阳性，18例EBNA1呈阳性，13例LMP-1抗原呈阳性。结果表明，幽门螺杆菌相关的NH2Cl可诱导潜伏感染EBV的胃上皮细胞发生EBV裂解转化。