与非癌性胃十二指肠组织相比，胃癌中爱泼斯坦-巴尔病毒的病毒载量更高。
Higher viral load of Epstein-Barr virus in gastric cancer compared with non-cancerous gastroduodenal tissues.
作者信息
Zebardast Arghavan, Pazhoohan Maryam, Cherati Azadeh Yazdani, Salehi Maryam, Amoli Saghar Saber, Yahyapour Yousef, Ranaee Mohammad, Shirvani Javad Shokri, Sadeghi Farzin
机构信息
Students Research Committee, Babol University of Medical Sciences, Babol, Iran.
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol; University of Medical Sciences, Babol, Iran.
出版信息
Infez Med. 2022 Jun 1;30(2):263-271. doi: 10.53854/liim-3002-12. eCollection 2022.
INTRODUCTION
Epstein-Barr Virus (EBV)-associated gastric cancer is a distinct molecular subtype of gastrointestinal carcinomas as defined by the Cancer Genome Atlas.
METHODS
In the present study 237 samples from Iranian patients diagnosed with gastric cancer and gastroduodenal disease were retrospectively examined for EBV infection by quantitative Real-Time PCR.
RESULTS
Of the 237 samples tested, EBV DNA was detected in 37 samples (15.6%), in 13 of the 81 gastric cancer cases (16%), and 24 of the 156 non-cancerous samples (15.4%). The EBV infection rate was found higher in patients with gastric ulcer (35%) and duodenal ulcer (21.9%) compared to patients with gastric cancer (16%) and gastritis (19.6%). The EBV-encoded small RNA (EBER) copy number in the gastric cancer group (mean = 2.14×10 with range of 2.14×10 to 4.10×10 copies/ cell) was higher than gastroduodenal diseases group (mean = 1.39×10 with range 1.11×10 to 2.35×10 copies/ cell), and this difference was statistically significant (P >0.001).
CONCLUSION
The higher number of copies of EBV-EBER in the gastric cancer group compared to the non-cancer group confirmed the possible role of EBV in inducing cancer.
引言
根据癌症基因组图谱的定义，爱泼斯坦-巴尔病毒（EBV）相关的胃癌是胃肠道癌的一种独特分子亚型。
方法
在本研究中，对237例被诊断为胃癌和胃十二指肠疾病的伊朗患者的样本进行回顾性研究，通过定量实时PCR检测EBV感染情况。
结果
在检测的237个样本中，37个样本（15.6%）检测到EBV DNA，其中81例胃癌病例中的13例（16%），以及156例非癌样本中的24例（15.4%）。与胃癌患者（16%）和胃炎患者（19.6%）相比，胃溃疡患者（35%）和十二指肠溃疡患者（21.9%）的EBV感染率更高。胃癌组中EBV编码的小RNA（EBER）拷贝数（平均值=2.14×10，范围为2.14×10至4.10×10拷贝/细胞）高于胃十二指肠疾病组（平均值=1.39×10，范围为1.11×10至2.35×10拷贝/细胞），且这种差异具有统计学意义（P>0.001）。
结论
与非癌组相比，胃癌组中EBV-EBER的拷贝数更高，证实了EBV在诱发癌症中可能发挥的作用。
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