Reeves Emer P, Nagl Markus, O'Keeffe Joseph, Kelly Judy, Kavanagh Kevin
Medical Mycology Unit, National Institute for Cellular Biotechnology, Department of Biology, NUI Maynooth, Co. Kildare, Ireland.
Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.
J Med Microbiol. 2006 Jul;55(Pt 7):913-918. doi: 10.1099/jmm.0.46405-0.
The fungistatic and fungicidal activity of N-chlorotaurine (NCT), a long-lived oxidant produced by stimulated neutrophils, was investigated. Physiological concentrations (75-100 microM) of NCT showed clear fungicidal activity against a range of Aspergillus isolates. Moreover, killing by NCT was significantly increased in the presence of ammonium chloride, explained by the formation of monochloramine by halogenation of ammonium. One clinical isolate of Aspergillus fumigatus was characterized for the production of the immunosuppressive agent gliotoxin, and NCT was shown to cause destruction of gliotoxin, possibly via reduction of the disulphide bridge. Because of its endogenous nature and its high antifungal activity, NCT appears to be a good choice for topical treatment of Aspergillus infections, and the results of this study further substantiate its therapeutic efficacy.
对刺激的中性粒细胞产生的一种长效氧化剂N-氯代牛磺酸（NCT）的抑菌和杀菌活性进行了研究。生理浓度（75-100微摩尔）的NCT对一系列曲霉菌分离株显示出明显的杀菌活性。此外，在氯化铵存在的情况下，NCT的杀菌作用显著增强，这可以通过氯化铵卤化形成一氯胺来解释。对一株烟曲霉临床分离株进行了免疫抑制剂gliotoxin产生情况的鉴定，结果表明NCT可能通过还原二硫键导致gliotoxin的破坏。由于其内源性本质及其高抗真菌活性，NCT似乎是局部治疗曲霉菌感染的一个不错选择，本研究结果进一步证实了其治疗效果。
