Chirkov Iu Iu, Belushkina N N, Tyshchuk I A, Severina I S
Biull Eksp Biol Med. 1991 Feb;111(2):152-4.
Activity of guanylate cyclase (GC) and its capacity for sodium nitroprusside (SNP) activation were determined in platelets with different state of aggregation. The development of ADP-induced reversible aggregation was accompanied by a decrease in the basal GC activity and by an increase in the SNP activation of GC. It was shown that elevation of GC sensitivity to SNP during the aggregation might be due to the decrease in the state of enzyme blood deficiency. Preincubation of platelets with SNP before ADP adding markedly diminished or even prevented aggregation, depending on SNP concentration. GC parameters in platelets with prevented aggregation were just the same as in control. It is suggested that the regulatory role of cGMP system in platelet aggregation may be seen in the increase in GC sensitivity to endogenous activator, presumably to NO.
测定了处于不同聚集状态的血小板中鸟苷酸环化酶（GC）的活性及其对硝普钠（SNP）的激活能力。ADP诱导的可逆聚集的发展伴随着基础GC活性的降低以及GC对SNP激活作用的增强。结果表明，聚集过程中GC对SNP敏感性的升高可能是由于酶血缺乏状态的降低。在加入ADP之前用SNP预孵育血小板，根据SNP浓度的不同，可显著减弱甚至阻止聚集。阻止聚集的血小板中的GC参数与对照中的相同。提示cGMP系统在血小板聚集中的调节作用可能表现为GC对内源性激活剂（可能是NO）敏感性的增加。
