Severina I S, Belushkina N N
Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
Biochem Int. 1992 Dec;28(4):621-31.
The dynamics of changes in the stimulation of human platelet guanylate cyclase by some activators in aggregating platelets was studied. It was shown that ADP-induced aggregation of human platelets (donors) is accompanied by the enhancement of the intensity of guanylate cyclase activation by sodium nitroprusside, L-arginine, protoporphyrin IX and arachidonic acid and also by the increase in cGMP content. Immediately after the induction of aggregation the intensity of guanylate cyclase activation and cGMP content begin to increase. The rise reaches its maxima within several minutes, then followed by a fall to the initial level. The peaks of the enhanced capacity for guanylate cyclase activation by the above compounds coincide in time and intensity. On the basis of the proposed hypothetical scheme of cGMP action as a regulator of platelet aggregation a possible mechanism of enhancing the capacity of guanylate cyclase to be stimulated by various activators in aggregating platelets is suggested.
研究了某些激活剂对聚集血小板中人类血小板鸟苷酸环化酶刺激作用的变化动态。结果表明，ADP诱导的人类血小板（供体）聚集伴随着硝普钠、L-精氨酸、原卟啉IX和花生四烯酸对鸟苷酸环化酶激活强度的增强以及cGMP含量的增加。聚集诱导后立即，鸟苷酸环化酶激活强度和cGMP含量开始增加。升高在几分钟内达到最大值，然后下降到初始水平。上述化合物增强鸟苷酸环化酶激活能力的峰值在时间和强度上相吻合。基于所提出的cGMP作为血小板聚集调节剂的假设作用机制，提出了一种在聚集血小板中增强鸟苷酸环化酶被各种激活剂刺激能力的可能机制。
