来自桑黄属真菌PL3的桑黄菌素A具有抗氧化活性。

Phellinsin A from Phellinus sp. PL3 exhibits antioxidant activities.

作者信息

Hwang Eui Il, Kim Ju-Ryoung, Jeong Tae-Sook, Lee Sangku, Rho Mun-Chual, Kim Sung Uk

机构信息

Agro-Tech Research Group, KT&G Central Research Institute, Daejeon 305-805, Korea.

出版信息

Planta Med. 2006 May;72(6):572-5. doi: 10.1055/s-2006-931533.

DOI:10.1055/s-2006-931533

PMID:16773545

Abstract

Phellinsin A, which was isolated from the culture broth of Phellinus sp. PL3, exhibited significant low-density lipoproteins (LDL)-antioxidant activity. It inhibited the Cu2+-mediated oxidation of LDL (IC50: 5.3 microM) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-mediated oxidation of LDL (IC50: 2.8 microM) in the thiobarbituric acid-reactive substances (TBARS) assay as well as the macrophage-mediated LDL oxidation (73% inhibition at 5 microM). In addition, it delayed LDL oxidation with a prolonged lag time (192 min at 2 microM, control: 44 min). This compound also showed a 10-fold more potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50: 1.7 microM) than trolox (IC50: 18.6 microM), a known DPPH inhibitor. In addition, phellinsin A inhibited xanthine oxidase activity with an IC50 value of 31.0 microM, whereas allopurinol, a xanthine oxidase inhibitor, showed an IC50 value of 40.7 microM.

摘要

从桑黄属菌株PL3的培养液中分离得到的桑黄菌素A表现出显著的低密度脂蛋白（LDL）抗氧化活性。在硫代巴比妥酸反应性物质（TBARS）分析中，它抑制了Cu2+介导的LDL氧化（IC50：5.3微摩尔）以及2,2'-偶氮二（2-甲基丙脒）二盐酸盐（AAPH）介导的LDL氧化（IC50：2.8微摩尔），同时也抑制了巨噬细胞介导的LDL氧化（5微摩尔时抑制率为73%）。此外，它延长了LDL氧化的滞后期（2微摩尔时为192分钟，对照为44分钟）。该化合物还表现出比已知的DPPH抑制剂曲洛昔康（IC50：18.6微摩尔）强10倍的1,1-二苯基-2-苦基肼基（DPPH）自由基清除活性（IC50：1.7微摩尔）。此外，桑黄菌素A抑制黄嘌呤氧化酶活性的IC50值为31.0微摩尔，而黄嘌呤氧化酶抑制剂别嘌醇的IC50值为40.7微摩尔。