日本桤木中二芳基庚烷类衍生物对人低密度脂蛋白氧化的抗氧化作用。

Antioxidant effects of diarylheptanoid derivatives from Alnus japonica on human LDL oxidation.

作者信息

Lee Woo Song, Kim Ju-Ryoung, Im Kyung-Ran, Cho Kyung-Hyun, Sok Dai-Eun, Jeong Tae-Sook

机构信息

National Research Lab of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology, Yousong, Daejeon, Republic of Korea.

出版信息

Planta Med. 2005 Apr;71(4):295-9. doi: 10.1055/s-2005-864093.

DOI:10.1055/s-2005-864093

PMID:15856403

Abstract

Two diarylheptanoids, oregonin (1) and hirsutanone (2), were isolated by bioassay-guided fractionation of the methanol extracts of the leaves of Alnus japonica Steud and their structures were elucidated from their spectroscopic data. Compounds 1 and 2 exhibited significant low-density lipoprotein (LDL)-antioxidant activities, e. g., thiobarbituric acid-reactive substances (TBARS) assay (1: IC50 = 3.2 microM, 2: IC50 = 1.5 microM), lag time (1 : 162 min, 2 : 230 min at 2.0 microM, control: 63 min), relative electrophoretic mobility (REM, inhibition of 1 : 71%, 2 : 75% at 10 microM), apoB-100 fragmentation (inhibition of 1 : 27.3%, 2 : 40.3% at 10 microM) and macrophage-mediated LDL oxidation (inhibition of 1 : 82%, 2 : 84% at 1 microM).

摘要

通过对日本桤木叶甲醇提取物进行生物活性导向分离，得到了两种二芳基庚烷类化合物，即俄勒冈宁（1）和绒毛润楠酮（2），并根据其光谱数据阐明了它们的结构。化合物1和2表现出显著的低密度脂蛋白（LDL）抗氧化活性，例如，硫代巴比妥酸反应性物质（TBARS）测定（1：IC50 = 3.2微摩尔，2：IC50 = 1.5微摩尔）、延迟时间（1：162分钟，2：在2.0微摩尔时为230分钟，对照：63分钟）、相对电泳迁移率（REM，在10微摩尔时1的抑制率为71%，2的抑制率为75%）、载脂蛋白B-100片段化（在10微摩尔时1的抑制率为27.3%，2的抑制率为40.3%）以及巨噬细胞介导的LDL氧化（在1微摩尔时1的抑制率为82%，2的抑制率为84%）。