Burdan F, Szumiło J, Dudka J, Dabrowski A, Maciejewski R, Wójtowicz Z
Experimental Teratology Unit of the Human Anatomy Department, Medical University of Lublin, Lublin, Poland.
Folia Morphol (Warsz). 2006 May;65(2):111-5.
Cathepsin D is a cysteine endopeptidase that belongs to the lysosomal enzyme family. The aim of the study was to evaluate the enzyme immunoexpression and activity in selected male genital organs in mature Wistar rats. The activity of cathepsin D was measured spectrophotometrically in homogenates of the testis, epididymis, seminal vesicle and prostate. Immunohistochemical staining was also performed in the ductus deferens. Enzyme activity was found in the following sequence: testis>epididymis>dorsal prostatic lobe>seminal vesicle>lateral prostatic lobe>ventral prostatic lobe. Although there were differences in enzyme activity between various organs of the male reproductive system, cathepsin D immunoreactivity was seen exclusively in the Sertoli and Leydig cells in the testis.
组织蛋白酶D是一种属于溶酶体酶家族的半胱氨酸内肽酶。本研究的目的是评估成熟Wistar大鼠选定雄性生殖器官中该酶的免疫表达和活性。采用分光光度法测定睾丸、附睾、精囊和前列腺匀浆中组织蛋白酶D的活性。还对输精管进行了免疫组织化学染色。发现酶活性顺序如下：睾丸>附睾>前列腺背叶>精囊>前列腺侧叶>前列腺腹叶。尽管雄性生殖系统各器官之间的酶活性存在差异，但组织蛋白酶D免疫反应仅在睾丸的支持细胞和间质细胞中可见。
