Asari M, Sasaki K, Miura K, Ichihara N, Nishita T
Department of Anatomy I, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Am J Vet Res. 1996 Apr;57(4):439-43.
To elucidate locations of cytosolic carbonic anhydrase isoenzyme (CA-I, CA-II, and CA-III)-positive epithelial cells in equine male reproductive organs.
Descriptive and immunohistochemical study.
4 clinically normal male horses.
The testis (seminiferous tubules, rete tubules), epididymis (initial, middle, and terminal segments), proximal and distal portions of the ductus deferens, ampulla ductus deferentis, seminal vesicle, prostate, and bulbourethral gland were excised from euthanatized horses after administration of an overdose of pentobarbital. The tissue specimens were quickly placed in fixative solution, dehydrated in ethanol, and embedded; then thin sections were cut. For immunohistochemical staining, antibodies against purified equine CA-I, CA-II, and CA-III were raised in rabbits. After examination of the specificity of each antiserum, the monospecific antisera against carbonic anhydrase isoenzymes were used to localize the isoenzymes.
Specific staining for CA-III was found in the Sertoli and basal cells of the ductus deferens. Most of the testicular and epididymal tissue, as well as ductus deferens, were virtually negative for the enzymes when stained with the antibody to CA-I and CA-II. In the initial segment of the epididymis, a few principal cells had intense cytoplasmic staining with anti-CA-II. In the male accessory glands, CA-I, CA-II, and CA-III were detected in the epithelial cells of the seminal vesicle, prostate, and bulbourethral gland.
In the equine male reproductive tract, the bicarbonate in semen originates mainly from accessory reproductive glands. All 3 isoenzymes may have central roles in the regulation of bicarbonate concentration in seminal plasm and, accordingly, regulate seminal plasma pH. Distribution of CA-III in Sertoli and basal cells of the ductus deferens suggests other specialized physiologic roles.
阐明胞质碳酸酐酶同工酶（CA-I、CA-II和CA-III）阳性上皮细胞在雄性马生殖器官中的位置。
描述性和免疫组织化学研究。
4匹临床正常的雄性马。
在过量给予戊巴比妥钠使马安乐死后，切除睾丸（生精小管、睾丸网）、附睾（起始段、中段和终末段）、输精管的近端和远端、输精管壶腹、精囊、前列腺和尿道球腺。将组织标本迅速置于固定液中，用乙醇脱水并包埋；然后切成薄片。对于免疫组织化学染色，用兔制备针对纯化马CA-I、CA-II和CA-III的抗体。在检测每种抗血清的特异性后，使用针对碳酸酐酶同工酶的单特异性抗血清来定位同工酶。
在输精管的支持细胞和基底细胞中发现了CA-III的特异性染色。当用抗CA-I和抗CA-II抗体染色时，大多数睾丸和附睾组织以及输精管对这些酶几乎呈阴性。在附睾起始段，一些主细胞用抗CA-II染色时细胞质有强烈染色。在雄性附属腺中，在精囊、前列腺和尿道球腺的上皮细胞中检测到了CA-I、CA-II和CA-III。
在雄性马生殖道中，精液中的碳酸氢盐主要来自附属生殖腺。所有3种同工酶可能在调节精浆中碳酸氢盐浓度方面发挥核心作用，并因此调节精浆pH值。CA-III在输精管支持细胞和基底细胞中的分布表明其有其他特殊的生理作用。
