Hale Matthew B, Nolan Garry P
Stanford University, Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, 269 Campus Drive, Stanford, CA 94305, USA.
Curr Opin Mol Ther. 2006 Jun;8(3):215-24.
Striving to achieve greater clinical relevance, researchers in basic science and in drug discovery are transitioning from biochemical investigations using cell lines to technologies that garner mechanistic information from primary patient material. Such studies can be broad in scope, despite limited sample material and cell-type heterogeneity. The development of flow cytometry for following intracellular signaling has met some of these demands and opened new avenues for mechanistic exploration. This review covers some of the most recent research to leverage this new technology and follows two new developments: increasing interest in JAK/STAT signaling, and experimental strategies that reveal disease-induced modulation of signaling networks.
为了实现更大的临床相关性，基础科学和药物研发领域的研究人员正从使用细胞系的生化研究转向从原发性患者材料中获取机制信息的技术。尽管样本材料有限且细胞类型存在异质性，但此类研究的范围可能很广。用于跟踪细胞内信号传导的流式细胞术的发展满足了其中一些需求，并为机制探索开辟了新途径。本综述涵盖了利用这项新技术的一些最新研究，并介绍了两个新进展：对JAK/STAT信号传导的兴趣日益增加，以及揭示疾病诱导的信号网络调节的实验策略。
