Yamade I, Ishiguro T, Seto A
Department of Obstetrics and Gynecology, Shiga University of Medical Sciences, Otsu, Japan.
J Med Virol. 1991 Apr;33(4):268-72. doi: 10.1002/jmv.1890330411.
The presence or absence of the anti-human T-cell leukemia virus type (HTLV-I) antibody and the HTLV-I proviral genome was examined in the offspring of inbred rabbits, which were born to HTLV-I carrier does. The results showed that not all offspring born to the carriers were infected and that not all the infected offspring seroconverted at the age of 10 weeks, which is similar to observations made in human carriers. The anti-HTLV-I antibody was assayed by indirect immunofluorescence in 55 offspring at the age of 10 weeks, which were born to B/J or (B/J x Chbb:HM)F1 seropositive HTLV-I carrier does. Twelve out of 31 offspring born from F1 x F1 mating were seropositive, whereas none of 24 offspring born from B/J x B/J mating, F1 x B/J mating, or F1 x Chbb:HM mating were seropositive. The polymerase chain reaction (PCR) method revealed the presence of the HTLV-I proviral genome in 18 out of 23 offspring born from F1 x F1 mating (F2 hybrids). In these 18 HTLV-I-infected F2 hybrids, 8 were seropositive and 10 were seronegative. The major histocompatibility complex (MHC) of these 23 F2 hybrids was analyzed by restriction fragment length polymorphism (RFLP) in southern hybridization. The results showed no close correlation of MHC with HTLV-I susceptibility or with seroconversion. Natural infection via mother-to-child transmission of virus seems to produce seronegative as well as seropositive carriers. This rabbit model may be useful for the study of seronegative virus carriers via mother-to-child transmission of HTLV-I.
对感染人类T细胞白血病病毒1型（HTLV-I）的近交系兔所产后代，检测其是否存在HTLV-I抗体及HTLV-I前病毒基因组。结果显示，并非所有携带病毒的母兔所产后代均被感染，且并非所有受感染后代在10周龄时都出现血清转化，这与人类携带者的情况相似。采用间接免疫荧光法检测了55只10周龄后代的抗HTLV-I抗体，这些后代由B/J或（B/J×Chbb:HM）F1血清阳性的HTLV-I携带母兔所生。F1×F1交配所生的31只后代中有12只血清阳性，而B/J×B/J交配、F1×B/J交配或F1×Chbb:HM交配所生的24只后代均无血清阳性。聚合酶链反应（PCR）方法显示，F1×F1交配所生的23只后代（F2杂种）中有18只存在HTLV-I前病毒基因组。在这18只感染HTLV-I的F2杂种中，8只血清阳性，10只血清阴性。通过Southern杂交中的限制性片段长度多态性（RFLP）分析了这23只F2杂种的主要组织相容性复合体（MHC）。结果显示，MHC与HTLV-I易感性或血清转化无密切相关性。通过母婴传播病毒的自然感染似乎会产生血清阴性和血清阳性携带者。该兔模型可能有助于研究HTLV-I母婴传播导致的血清阴性病毒携带者。
