Seto A, Kawanishi M, Matsuda S, Ogawa K
Department of Microbiology, Shiga University of Medical Science, Otsu, Japan.
J Exp Med. 1988 Dec 1;168(6):2409-14. doi: 10.1084/jem.168.6.2409.
Six HTLV-I-transformed T cell lines were prepared from PBL of three rabbits each of B/J and Chbb:HM strains, and were inoculated into newborn rabbits of these two strains, and of their F1 hybrid. None of three B/J cell lines induced anti-HTLV-I antibody response in newborn B/J rabbits, whereas all three Chbb:HM cell lines did induce a response in newborn Chbb:HM rabbits. These B/J cell lines however could induce antibody response in adult B/J as well as newborn Chbb:HM rabbits, and a Chbb:HM cell line could induce a response in a newborn B/J rabbit. Similar unresponsiveness was observed in (B/J x Chbb:HM)F1 hybrids neonatally inoculated with B/J cells. Unresponsiveness was abrogated by reinoculation of some but not other cell lines. Viral antigen-positive cell lines harboring HTLV-I provirus genomes were established from such seronegative B/J and F1 rabbits. Simultaneous inoculation of HTLV-I-transformed cells and SPV resulted in the induction of papilloma and antibody against SPV, but not antibody against HTLV-I. The present findings thus reveal that neonatal infection of HTLV-I could result in immunological tolerance to the virus antigens, thereby leading to a persistent infection without antibody induction.
从B/J和Chbb:HM品系的三只兔子的外周血淋巴细胞（PBL）中分别制备了6株HTLV-I转化的T细胞系，并将其接种到这两个品系及其F1杂种的新生兔体内。三个B/J细胞系在新生B/J兔中均未诱导出抗HTLV-I抗体反应，而所有三个Chbb:HM细胞系在新生Chbb:HM兔中均诱导出了反应。然而，这些B/J细胞系能够在成年B/J兔以及新生Chbb:HM兔中诱导抗体反应，并且一个Chbb:HM细胞系能够在新生B/J兔中诱导出反应。在用B/J细胞系进行新生儿接种的（B/J×Chbb:HM）F1杂种中也观察到了类似的无反应性。再次接种某些但不是其他细胞系可消除无反应性。从这种血清阴性的B/J和F1兔中建立了携带HTLV-I前病毒基因组的病毒抗原阳性细胞系。同时接种HTLV-I转化细胞和乳头瘤病毒（SPV）可诱导乳头瘤形成以及针对SPV的抗体，但不能诱导针对HTLV-I的抗体。因此，目前的研究结果表明，HTLV-I的新生儿感染可能导致对病毒抗原的免疫耐受，从而导致持续感染而不诱导抗体产生。
